Communication
ChemComm
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In conclusion, we have presented a far-red emissive, two-
photon NRNO probe with an adequate TP cross section and a
far-red emission band centred at 650 nm which can selectively
label dU with treatment by UNG. Given the rapidly expanding
interest in the role of dU in biological functions, this probe
offers a reliable, fast, cheap and easy method to gain quantita-
tive and qualitative information about uracil levels in DNA.
Furthermore, this simple assay can also be applied to the
analysis of X sites in genomic DNA and provides a reference
and tool for future investigations of deoxyuridine modification.
This work was financially supported by the National Natural
Science Foundation of China (NSFC) projects (21822704,
21778040 and 91940304 to X. W.; 22037004, 91753201 and
21721005 to X. Z.; 21778041 to F. W.). We thank Prof. Chengqi
Yi (Peking University) who provided the HEK-293T cell line that
stably expresses UNG2.
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Conflicts of interest
There are no conflicts to declare.
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This journal is The Royal Society of Chemistry 2021
Chem. Commun., 2021, 57, 2784À2787 | 2787