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for 30 min at 37 ꢀC with FTase enzyme (16.6 nM), [3H]-farne-
sylpyrophosphate(FPP) (0.3 mM, 20 Ci/mmol, Dupont NEN)
and serially diluted test compounds at given concentration in
the buffer containing 50 mM HEPES, pH 7.4, 250 mM MgCl2,
25 mM KCl, 0.5% N-octylglucoside, 50 mM ZnCl2, and 10 mM
DTT. The enzyme reactions were then quenched by adding the
solution containing 10% HCl in absolute ethanol. The [3H]
FPP-incorporated Ras was collected by filter binding (25 mm
Glass fiber filter, Whatman) and quantitated by scintillation
counter.
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FT IC50: the concentration of compound required to reduce
the FTase-catalyzed incorporation of [3H]FPP into H-Ras
protein by 50%.