Tetrahedron p. 6603 - 6622 (1999)
Update date:2022-08-05
Topics:
Milecki, Jan
Zamaratski, Edouard
Maltseva, Tatiana V.
Foeldesi, Andras
Adamiak, Ryszard W.
Chattopadhyaya, Jyoti
A complete synthetic protocol as well as 1H- and 13C-NMR data on the monomer building blocks used for the solid-phase synthesis of specifically 13C-labelled (99 atom % 13C) stem (27A and 43G), bulge (24C) and loop (31U) regions of 29mer HIV-1 TAR RNA hairpin starting from the 13C6-D-glucose are presented. The complex NMR spectra of 13C-labelled monomer building blocks, due to the interaction of various 13C and 1H spins, have been assigned. It has been demonstrated by heteronuclear 2D NMR that the non-uniform labelling of the HIV-1 TAR 29mer RNA achieved herein by chemical synthesis provides an optimal opportunity to perform full T1 and T2 relaxation measurements (the 'NMR Relaxation Window') of each type of sugar-carbons for all four strategically placed 13C-labelled residues in a unique and unprecedented manner because of minimal overlap of 13C resonances compared to uniformly labelled oligo-RNA.
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Doi:10.1021/jo00830a051
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