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ChemComm
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COMMUNICATION
Journal Name
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Fig. 5 Confocal fluorescence imaging for ClO- detection in HeLa cells using NBD-DOP. (a-
c) images of HeLa cells incubated with NBD-DOP for 20 min; (d-f) images of HeLa cells
after treatment with NBD-DOP (5 μM) for 20 min, and subsequent treatment with NaOCl
(50 μM) for 20 min; (g-i) images of HeLa cells incubated with LPS (2 μg/mL) for 24 h,
further incubated with PMA (2 μg/mL) and then with NBD-DOP (2 μM) for 20 min.
that NBD-DOP is capable of detecting exogenous or
endogenous HOCl in living cells.
To conclude, we have synthesized a facile fluorescent probe
NBD-DOP to detect HOCl. NBD-DOP can be obtained through
one-step synthesis. The dopamine moiety has dual functions: 1.
The ortho-benzenediol group of dopamine plays PET effect on the
NBD fluorophore. This provides the weak fluorescence background
of NBD-DOP and high fluorescence enhancement after reacting with
HOCl; 2. The dopamine group could react with HOCl specifically and
efficiently. Our data supports that NBD-DOP functions as a practical
fluorescent probe that features fast response (within 30 s), high
selectivity and sensitivity to HOCl, and wide pH range in applications.
Importantly, NBD-DOP has been successfully applied for imaging
exogenous or endogenous HOCl in living cells.
This work was supported by the National Natural Science
Foundation of China (No. 21602033 and 21471037), Guangxi
Natural Science Foundation (2016GXNSFBA380123), Shenzhen
Basic Research Project (JCYJ20160601173218804), Guangdong
Natural Science Funds for Distinguished Young Scholars
(15ZK0307) and Science and Technology Planning Project of
Guangdong Province (2017A050506051).
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Conflicts of interest
There are no conflicts to declare.
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4 | J. Name., 2012, 00, 1-3
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