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bond of the substrate; the lack of this atom may be
responsible for unusual bindingmodes of simpler phos-
phonic acid inhibitors. Unexpectedly, the D analogue is
a significantly better inhibitor of YopH than the natural
L isomer, suggesting that stereochemistry of such com-
pounds may offer an avenue for exploitation.
Acknowledgements
This work was supported by NIH Grant GM47297 to
A.C.H. and an Undergraduate Research and Creative
Opportunities (URCO) grant from USU to J.M.Y. We
thank Professor John Denu for PP2C, and Frank Rus-
nak (deceased) for kPP. The authors gratefully acknowl-
edge the use of equipment in the Shimadzu Analytical
Sciences Laboratory at Utah State University, created
with equipment donated by Shimadzu Scientific Instru-
ments Inc. (Columbia, MD).
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