Article
J. Agric. Food Chem., Vol. 58, No. 11, 2010 6677
(6) Munday, R.; Mhawech-Fauceglia, P.; Munday, C. M.; Paonessa,
J. D.; Tang, L.; Munday, J. S.; Lister, C.; Wilson, P.; Fahey, J. W.;
Davis, W.; Zhang, Y. Inhibition of urinary bladder carcinogenesis by
broccoli sprout. Cancer Res. 2008, 68, 1593–1600.
(7) Taylor, D. N.; Parsonnet, J. Epidemiology and natural history of
H. pylori infections. In Infections of the Gastrointestinal Tract; Blaser,
M. J., Smith, P. F., Ravdin, J., Greenberg, H., Guerrant, R. L., Eds.; Raven
Press: New York, NY, 1995; pp 551.
of broccoli using mass spectral fingerprinting and analysis of
variance-principal component analysis. J. Agric. Food Chem.
2008, 56, 9819–9827.
(25) Lin, L.-Z.; Marnly, J. M. Identification of the phenolic components
of collard greens, kale, and Chinese broccoli. J. Agric. Food Chem.
2009, 57, 7401–7408.
(26) Liang, H.; Yuan, Q. P.; Dong, H. R.; Liu, Y. M. Determination of
sulforaphane in broccoli and cabbage by high-performance liquid
chromatography. J. Food Compos. Anal. 2006, 19, 473–476.
(27) Chiang, W. C. K.; Pusateri, J.; Leitz, R. E. A. Gas chromatography/
mass spectrometry method for the determination of sulforaphane
and sulforaphane nitrile in broccoli. J. Agric. Food Chem. 1998, 46,
1018–1021.
(8) Dunn, B. E.; Cohen, H.; Blaser, M. J. Helicobacter pylori. Clin.
Microbiol. Rev. 1997, 10, 720-741.
(9) Frenck, R. W.; Clemens, J. Helicobacter in the developing world.
Microbes Infect. 2003, 5, 705–713.
(10) Peitz, U.; Menegatti, M.; Vaira, D.; Malfertheiner, P. The European
meeting on Helicobacter pylori: therapeutic news from Lisbon. Gut
1998, 43, S66–69.
(11) Ahn, Y. J.; Park, S. J.; Lee, S. G.; Shin, S. C.; Choi, D. H.
Cordycepin: selective growth inhibitor derived from liquid culture
of Cordyceps militaris against Clostridium spp. J. Agric. Food Chem.
2000, 48, 2744–2748.
(28) Park, B. S.; Lee, H. K.; Lee, S. E.; Piao, X. L.; Takeoka, G. R.;
Wong, R. Y.; Ahn, Y. J.; Kim, J. H. Antibacterial activity of
Tabebuia impetiginosa Martius ex DC (Taheebo) against Helicobac-
ter pylori. J. Ethnopharmacol. 2006, 105, 255–262.
(29) Fahey, J. W.; Haristoy, X.; Dolan, P. M.; Kensler, T. W.; Scholtus,
I.; Stephenson, K. K.; Talalay, P.; Lozniewski, A. Sulforaphane
inhibits extracellular, intracellular, and antibiotic-resistant strains of
Helicobacter pylori and prevents benzo[a]pyrene-induced stomach
tumors. Proc. Natl. Acad. Sci. U.S.A. 2002, 99, 7610–7615.
(30) Nakagawa, K.; Umeda, T.; Higuchi, O.; Tsuzuki, T.; Suzuki, T.;
Miyazawa, T. Evaporative light-scattering analysis of sulforaphane
in broccoli samples: quality of broccoli products regarding sulfor-
aphane contents. J. Agric. Food Chem. 2006, 54, 2479–2483.
(31) Chiang, W. C. K.; Pusateri, D. J.; Leitz, R. E. A. Gas chromato-
graphy/mass spectrometry method for the determination of sulfor-
aphane and sulforaphane nitrile in broccoli. J. Agric. Food Chem.
1998, 46, 1018–1021.
(32) Johansson, N. L.; Pavia, C. S.; Chiao, J. W. Growth inhibition of a
spectrum of bacterial and fungal pathogens by sulforaphane, an
isothiocyanate product found in broccoli and other cruciferous
vegetables. Planta Med. 2008, 74, 747–750.
(33) Haristoy, X.; Fahey, J. W.; Scholtus, I.; Lozniewski, A. Evaluation
of the antimicrobial effects of several isothiocyanates on Helico-
bacter pylori. Planta Med. 2005, 71, 326–330.
(34) Maeda, T.; Maekawa, K.; Suzuki, T.; Oosawa, K.; Ohshima, C.
Supplemental light enhances polyphenol content and antioxidative
capacity of broccoli sprouts grown in indoor production system.
Acta Hortic. 2008, 765, 217–223.
(35) Shign, J.; Upadhyay, A. K.; Singh, S.; Rai, M. Total phenolics
content and free radical scavenging activity of Brassica vegetables.
J. Food Sci. Technol. 2009, 46, 595–597.
(36) Kaur, C.; Kumar, K.; Anil, D.; Kapoor, H. C. Variations in
antioxidant activity in broccoli (Brassica oleracea L.) cultivars.
J. Food Biochem. 2007, 31, 621–638.
(37) Zhu, H.; Jia, Z.; Strobl, J.; Ehrich, M.; Misra, H. P.; Li, Y. Potent
induction of total cellular and mitochondrial antioxidants and phase
2 enzymes by cruciferous sulforaphane in rat aortic smooth muscle
cells: cytoprotection against oxidative and elctrophilic stress. Cardio-
vasc. Toxicol. 2008, 8, 115–125.
(12) Zoppi, G.; Cinquetti, M.; Benini, A.; Bonamini, E.; Minelli, E. B.
Modulation of the intestinal ecosystem by probiotics and lactulose in
children during treatment with ceftriaxone. Curr. Ther. Res. Clin.
Exp. 2001, 62, 418–435.
(13) Megraud, F. Resistance of Helicobacter pylori to antibiotics. In
´
Clinical Pharmacology and Therapy of Helicobacter pylori Infection;
Scarpignato, C., Bianchi, P. G., Eds.; Karger: Basel, Switzerland, 2004;
Vol. 11, pp 364.
(14) Moreno, D. A.; Carvajal, M.; Lopez-Berenguer, C.; Garcia-Viguera,
C. Chemical and biological characterization of nuraceutical com-
pounds of broccoli. J. Pharm. Biomed. Anal. 2006, 41, 1508–1522.
(15) Vasanthi, H. R.; Mukherjee, S.; Das, D. K. Potential health benefits
of broccoli - a chemico-biological overview. Mini-Rev. Med. Chem.
2009, 9, 749–759.
(16) Yanaka, A.; Fahey, J. W.; Fukumoto, A.; Nakayama, M.; Inoue, S.;
Zhang, S.; Tauchi, M.; Suzuki, H.; Hyodo, I.; Yamamoto, M.
Dietary sulforaphane-rich broccoli sprouts reduce colonization
and attenuate gastritis in Helicobacter-infected mice and humans.
Cancer Prev. Res. 2009, 2, 353–360.
(17) Haristory, X.; Angioi-Duprez, K.; Duprez, A.; Lozniewski, A.
Efficacy of sulforaphane in eradicating Helicobacter pylori in human
gastric xenografts implanted in nude mice. Antimicrob. Agents
Chemother. 2003, 47, 3982–3984.
(18) Brorowski, J.; Szajdek, A.; Borowska, E. J.; Ciska, E.; Zielinski, H.
Content of selected bioactive components and antioxidant properties
of broccoli (Brassica oleracea L.). Eur. Food Res. Technol. 2008, 226,
459–465.
(19) Kaur, C.; Kumar, K.; Anil, D.; Kappor, H. C. Variations in
antioxidant activity in broccoli (Brassica oleracea L.) cultivars.
J. Food Biochem. 2007, 31, 621–638.
(20) Mrkic, V.; Cocci, E.; Dalla Rosa, M.; Saccheitti, G. Effect of dry-
ing conditions on bioactive compounds and antioxidant activity
of broccoli (Brassica oleracea L.). J. Sci. Food Agric. 2006, 86,
1559–1566.
(21) Lee, J. J.; Shin, H. D.; Lee, Y. M.; Kim, A. R.; Lee, M. Y. Effect of
broccoli sprouts on cholesterol-lowering and anti-obesity effects in
rats fed high fat diet. K. K. Han’guk Sikp’um yongyang Kwahak
Hoechi 2009, 38, 309–318.
(22) Eberhardt, M. V.; Kobira, K.; Keck, A.-S.; Juvik, J. A.; Jeffery, E. H.
Correlation analyses of phytochemical composition, chemical, and
cellular measures of antioxidant activity of broccoli (Brassica
oleracea L. var. italica). J. Agric. Food Chem. 2005, 53, 7421–7431.
(23) Tian, Q.; Rosselot, R. A.; Schwartz, S. J. Quantitative determination
of intact glucosinolates in broccoli, broccoli sprouts, Brussels
sprouts, and cauliflower by high-performance liquid chromatogra-
phy-electrospray ionization-tandem mass spectrometry. Anal.
Biochem. 2005, 343, 93–99.
(38) Agrawal, S.; Winnik, B.; Buckley, B.; Mi, L.; Chung, F.-L.; Cook,
T. J. Simultaneous determination of sulforaphane and its major
metabolites from biological matrices with liquid chromatography-
tandem mass spectroscopy. J. Chromatogr., B 2006, 840, 99–107.
(39) Hue, R.; Hebbar, V.; Kim, B. R.; Chen, C.; Winnik, B.; Buckley, B.;
Soteropoulos, P.; Tolias, P.; Hart, R. P.; Kong, A. N. In vivo
pharmackokinetics and regulation of geneexpression profiles by
isothiocyanate sulforaphane in the rat. J. Pharmacol. Exp. Ther.
2004, 310, 263–271.
(40) Do, D. P.; Pai, S. B.; Rizvi, S. A. A.; D’Souza, M. J. Development of
sulforaphane encapsulated microspheres for cancer epigenetic ther-
apy. Int. J. Pharm. 2010, 386, 114–121.
Received for review January 27, 2010. Revised manuscript received
March 18, 2010. Accepted April 27, 2010.
(24) Luthria, D. L.; Lin, L.-Z.; Robbins, R. J.; Finley, J. W.; Banuelos,
G. S.; Harnly, J. M. Discriminating between cultivars and treatments