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575
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stroke, and irradiation treated lymphoma [10,15,25,26]. The NST-
732 (2) showedhigh sensitivity and specificityin targeting apoptotic
cells in vivo in all three models used. Uptake of NST-732 (2) in
apoptotic cells was higher than in the non-apoptotic ones, and the
specificity of NST-732 (2) targeting was demonstrated by its locali-
zation in regions of apoptotic/necrotic cell death, detected morpho-
logically and by TUNEL staining. To date, only fluorine-18 labeled
dansyl derivatives have been reported. In this work, DNSBA was de-
signed to facilitate radioiodine labeling which could be used for both
SPECT and PETimaging. Ourresults clearlyshow that 4 detects apop-
totic cancer cells induced by treatment with chemotherapeutic
drugs such as paclitaxel. In these experiments, we found that 4 does
not distinguish early stage apoptosis from late-stage apoptosis and
could be used to detect all stage of apoptotic cells. Moreover, this
does not affect their potential use of the reagent in evaluation treat-
ment efficacy since both early and late apoptotic cells are indicative
of positive signals of effective treatment. Because of its inherent
fluorescence feature, the DNSBA can be convenient used for in vivo
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cancer cells, and exhibits intracellular uptake and accumulation in
apoptoticcells. ThoughDNSBAcouldnotdifferentiatetheearly stage
and final stage of apoptosis from necrosis, it shows the ability to de-
tect multiple pathways leading to cell death may be advantageous in
the evaluation of cancer treatment indicative of a positive therapeu-
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broaden scope of the detection when compared with gold standard-
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a
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a
a
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(2008) 68–71.
This research was supported by the Program of New Century
Excellent Talents in University (NCET-09-0800), National Natural
Science Foundation of China (No. 30900377), and the Physician’s
Medical Education and Research (PMERF).
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molecule detector of apoptosis, Apoptosis 14 (2009) 257–267.
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