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ChemComm
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COMMUNICATION
Journal Name
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G. Markiewicz, D. Pakulski, A. Galanti, VD. POaI:t1r0o.n10ia3k9,/AC.9CCiCe0si3e1l0s6kEi,
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Fig. 4. OPM and TPM images of HepG2 cells incubated with AN-acetate
(10 μM) and AN-Br-acetate (10 μM) for 30 min. For the inhibition test,
HepG2 cells were pretreated with BNPP (500 μM) and AEBSF (2 mM) for
30 min and then incubated with the probes. Scale bars = 50 μm.
HepG2 cells were pretreated with bis(4-nitrophenyl) phosphate
(BNPP)
and
4-(2-aminoethyl)benzenesulfonyl
fluoride
17 X. He, Z. Zhao, L. Xiong, P. F. Gao, C. Peng, R. S. Li, Y. Xiong, Z.
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hydrochloride (AEBSF) for 30 min and were then incubated with
the probes, in which BNPP and AEBSF inhibited the enzymatic
hydrolysis by esterase. The HepG2 cells pretreated with BNPP
and AEBSF showed a lower fluorescence intensity than
untreated HepG2 cells. To check the photostability of the
probes, the fluorescence intensity of the probes was
investigated according to imaging time (Fig. S9). The
photostability of probes in cells was investigated by continuous
exposure to laser sources with 488 (for OP) and 900 nm (for TP).
The fluorescence intensity of both probes did not significantly
change in HepG2 cells after 60 min irradiation, demonstrating
the high photostablility of the probes. The small increase in the
fluorescence using OP was presumed to result from the
autofluorescence.
In summary, we demonstrated the unusual fluorescence
generated by AIE-active o-PAN luminogens. The o-PANs had a
TPA property, enabling long wavelength (900 nm) excitation,
which would be beneficial for biological cell imaging. The AN-Br-
acetate and AN-acetate probes showed high selectivity and
sensitivity toward esterase, by which they can be considered as
potential candidates for esterase enzyme detection and TP cell
imaging. This work was supported by the National Research
Foundation (NRF) of Korean government through Basic Science
Research Program (2018R1A2A2A14022019).
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Conflicts of interest
There are no conflicts to declare.
Notes and references
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4 | J. Name., 2012, 00, 1-3
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