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Emission Wavelenght (nm)
Fig. 15. Effects of sodium citrate upon KLK3 intrinsic fluorescence. The emission
spectra of KLK3 (2 M) in 50 mM Tris, pH 7.8 was evaluated before and with
increasing of sodium citrate concentration. The fluorescence measurements were
l
done at kex = 270 nm.
The hydrolysis of angiotensin I or II only at the Y–I bond is in
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Conclusions
The large activation of KLK3 by sodium citrate, which is concen-
trated in prostate and seminal fluid in high amount, indicates that
this salt can modulate the KLK3 activity in this organ. Although
KLK3 hydrolyses with high efficiency substrates at aromatic amino
acids, in the presence of sodium citrate the hydrolysis at R, Q and
particularly P the hydrolytic activities of KLK3 are also efficient. Fi-
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Acknowledgments
This work was supported in Brazil by Fundação de Amparo à
Pesquisa do Estado de São Paulo (FAPESP) and Conselho Nacional
de Desenvolvimento Científico e Tecnológico (CNPq). M.B. was sup-
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