Journal of Agricultural and Food Chemistry
Article
originate.44,45 Considering the chemical structure of the
synthesized oligosaccharides, lactulosucrose and its derivatives
could potentially be employed as prebiotic oligosaccharides that
could effectively combine the bioactive properties attributed to
lactulose and the possibility to possess lower fermentation rates.
This latter property would increase its interest as prebiotic by
increasing its capacity to reach the distal parts of the colon.
Finally, the transferred glucose moiety is attached to lactulose
through a α-(1→2) linkage which has shown high resistance to
in vitro and in vivo gastrointestinal digestion.46−48
(9) Mussatto, S. I.; Mancilha, I. M. Non-digestible oligosaccharides:
A review. Carbohydr. Polym. 2007, 68, 587−597.
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ASSOCIATED CONTENT
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(13) Mizota, T.; Mori, T.; Yaeshima, T.; Yanagida, T.; Iwatsuki, K.;
Ishibashi, N.; Tamura, Y.; Fukuwatari, Y. Effects of low dosages of
lactulose on the intestinal function of healthy adults. Milchwissenschaft
2002, 57, 311−315.
S
* Supporting Information
1H and 13C NMR, gCOSY, TOCSY, gHSQC, gHMBC, and
ROESY spectra of lactulosucrose. This material is available free
(14) Tuohy, K. M.; Ziemer, C. J.; Klinder, A.; Knobel, Y.; Pool-Zobel,
̈
B. L.; Gibson, G. R. A human volunteer study to determine the
prebiotic effects of lactulose powder on human colonic microbiota.
Microb. Ecol. Health Dis. 2002, 14, 165−173.
AUTHOR INFORMATION
Corresponding Author
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(15) Venema, K.; van Nuenen, M. H. M. C.; van den Heuvel, E. G.;
Pool, W.; van der Vossen, J. M. B. M. The effect of lactulose on the
composition of the intestinal microbiota and short-chain fatty acid
production in human volunteers and a computer-controlled model of
the proximal large intestine. Microb. Ecol. Health Dis. 2003, 15, 94−
105.
(16) Bass, P.; Dennis, S. The laxative effects of lactulose in normal
and constipated subjects. J. Clin. Gastroenterol. 1981, 3, 23−28.
(17) Pontes, F. A.; Silva, A. T.; Cruz, A. C. Colonic transit times and
the effect of lactulose or lactitol in hospitalized-patients. Eur. J.
Gastroenterol. Hepatol. 1995, 7, 441−446.
(18) Miller, M. A.; Parkman, H. P.; Urbain, J. L. C.; Brown, K. L.;
Donahue, D. J.; Knight, L. C.; Maurer, A. H.; Fisher, R. S. Comparison
of scintigraphy and lactulose breath hydrogen test for assessment of
orocecal transit - Lactulose accelerates small bowel transit. Dig. Dis. Sci.
1997, 42, 10−18.
(19) Wutzke, K. D.; Heine, W. E.; Plath, C.; Leitzmann, P.; Radke,
M.; Mohr, C.; Richter, I.; Gulzow, H. U.; Hobusch, D. Evaluation of
oro-coecal transit time: a comparison of the lactose-[13C, 15N]ureide
13CO2- and the lactulose H2-breath test in humans. Eur. J. Clin. Nutr.
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Funding
This work has been financed by Projects AGL2011-27884 and
Consolider Ingenio 2010 FUN-C-FOOD CSD2007-00063
both from Ministerio de Ciencia e Innovacion and project
́
POII10-0178-4685 from Junta de Comunidades de Castilla-La
Mancha and European Regional Development Fund (ERDF).
M.D.-M. thanks CSIC for a JAE predoctoral fellowship. M.H.
́
thanks MICINN for his “Ramon y Cajal” contract.
Notes
The authors declare no competing financial interest.
ACKNOWLEDGMENTS
The authors thank Paula Copovi for technical assistance in the
preliminary experiments.
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