176
M. KALUZHNAYA et al.
L
OUIS, P., TRUPER, H. G., GALINSKI, E. A.: Survival of Escherichia
coli during drying and storage in the presence of compatible
solutes. Appl. Microbiol. Biotechnol. 41, 684–688 (1994).
UFT, J. H.: Electron microscopy of cell extrageneous coats as re-
vealed by ruthenium red staining. J. Cell Biol. 23, 54A–55A
(1964).
CDONALD, I. R., KENNA, E. M., MURRELL, J. C.: Detection of
methylotrophic bacteria in enviromental samples with the
PCR. Appl. Environ. Microbiol. 61, 116–121 (1995).
IGUEZ, C. B., BOURGUE, D., GREEN, C. W., GROLEAU, D.: Detection
and isolation of methanotrophic bacteria possessimg soluble
methane monooxygenase (sMMO) genes using the polymerase
chain reaction (PCR). Microbiol. Ecol. 33, 21–31 (1997).
AMSARAEV B. B. ZHILINA, T. N., KULYROVA, A. B., GORLENKO, V.
M.: Methanogenesis in soda lakes of south eastern Siberia.
Mikrobiologiya. (English translation). 68, 671–676 (1999).
ASH T.: The colorimetric estimation of formaldehyde by means
of the Hantsch reaction. Biochem. J. 55, 416–421 (1953).
REMLAND, R. S., MARSH, L., DESWARAIS, D. L.: Methanogenesis
in Big Soda Lake. Appl. Environ. Microbiol. 43, 462–468
(1982).
References
BODROSSY, L., MURRELL, J. C., DALTON, H., KALMAN, M., PUSKAS,
L
L. G., KOVACS, K. L.: Heat-tolerant methanotrophic bacteria
from the hot-water effluent of a natural-gas field. Appl. Envi-
ron. Microbiol. 61, 3549–3555 (1995).
M
B
OWMAN, J. P., SLY, L. I., NICHOLS, P. D., HAYWARD, A. C.: Re-
vised taxonomy of the methanotrophs: description of Methy-
lobacter gen.nov., emendation of Methylococcus, validation of
Methylosinus and Methylocystis species, and a proposal that
M
the family Methylococcaceae includes only the group
methanotrophs. Int. J. Syst. Bacteriol. 43, 735–753. (1993).
I
BOWMAN, J. P., SLY, L. I., STACKEBRANDT, E.: The phylogenetic po-
N
sition of the family Methylococcaceae. Int. J. Syst. Bacteriol.
45, 182–185 (1995).
C
OLLINS, M. O.: Analysis of isoprenoid quinones, p. 329–366. In:
Methods in Microbiology (G. Gottschalk, ed.) New York,
Acad Press, 18 (1985).
N
O
E
DWARDS, U., ROGALL, T., BLOCKER, H., EMDE, M. and BOTTGER
,
E. C.: Isolation and direct complete nucleotide determination
of entire genes. Characterization of a gene coding for 16S ribo-
somal RNA. Nucleic. Acids. Res. 17, 7843–7853 (1989).
P
HELPS, P. A., AGARWAL, S. K., SPEITEL, G. E., GEORGIOU, G.:
Methylosinus trichosporium OB3b mutants having constitu-
tive expression of soluble methane monooxygenase in the pres-
ence of high levels of copper. Appl. Environ. Microbiol. 58,
3701–3708 (1992).
F
ELSENSTEIN, J.: PHYLIP (phylogenetic interference package) ver-
sion 3.5c. Department of Genetics, University of Washington,
Seattle, USA (1993).
G
RANT, W. D., JONES, B. E.: Alkaline environments, p. 126–133.
In: Encyclopedia of Microbiology. (J. Lederberg, ed). 2nd edi-
tion. San Diego, Acad. Press, 3 (2000).
P
IRT, J. S.: Principles of Microbe and Cell Cultivation. Oxford,
Blackwell Scientific Publications. (1995).
SAMBROOK, J., FRITSCH, E. F., MANIATIS, T.: Molecular Cloning. A
Laboratory Manual. (N. Irwin et al., eds). 2nd edition. New
York, Cold Spring Harbor Laboratory Press. (1989).
AUNDERS, S. E., BURKE. J. F.: Rapid isolation of miniprep DNA
for double strand sequencing. Nucleic Acids Res, 18,
4948–4948. (1990).
H
H
ANSON, R. S., HANSON, T. E.: Methanotrophic bacteria. Micro-
biol. Rev. 60, 437–471 (1996).
OLMES, A. J., COSTELLO, A., LIDSTROM, M. E., MURRELL, J. C.:
Evidence that particulate methane monooxygenase and ammo-
nia monooxygenase may be evolutionarily related. FEMS Mi-
crobiol. Lett. 132, 203–208 (1995).
S
S
OROKIN, D. Y., JONES, B. E., KUENEN, J. G.: A novel obligately
methylotrophic, methane-oxidizing Methylomicrobium species
J
EFFRIES, P., WILKINSON J. F.: Electron microscopy of cell wall
complex of Methylomonas albus. Arch. Microbiol. 119,
227–229 (1978).
from
a highly alkaline environment. Extermophiles. 4,
145–155 (2000).
S
IEBURTH, J. M., JOHNSON, P. W., EBERHARDT, M. A., SIERACKI, M.
E., LIDSTROM, M. E., LAUX, D.: The first methane-oxidizing bac-
terium from the upper mixing layer of the deep ocean: Methy-
lomonas pelagica sp. nov. Curr. Microbiol. 14, 285–293 (1987).
ROTSENKO, Y. A.: Isolation and characterization of obligate
methanotrophic bacteria, p. 329–336. In: Microbial produc-
tion and utilization of gases (H2, CO2, CH4). (H. G. SCHLEGEL
et al., eds). Goettingen, Goltze. (1976).
ROTSENKO, Y. A., DORONINA, N. V., GOVORUKHINA, N. I.:
Metabolism of non-motile obligately methylotrophic bacteria.
FEMS Microbiol. Letters 33, 293–297 (1986).
ROTSENKO, Y. A., KHMELENINA, V. N.: Extremophilic and ex-
tremotolerant methanotrophic bacteria. Arch. Microbiol. (sub-
mitted).
ROTSENKO, Y. A., SHISHKINA, V. N.: Studies on phosphate
metabolism in obligate methanotrophs. FEMS Microbiol. Rev.
87, 267–271 (1991).
HITTENBURY, R., DAVIES, S. L., DAWEY, J. F.: Exospores and cysts
formed by methane-utilizing bacteria. J. Gen. Microbiol. 61,
219 (1970).
AVARZIN, G. A., ZHILINA, T. N., KEVBRIN, V. V.: The alkalipilic
community and its functional diversity. Mikrobiologiya. (En-
glish translation). 68, 503–521 (1999).
J
OYE, S. B., CONNELL, T. L., MILLER, L. G., OREMLAND, R. S., JEL-
LISON, R. S.: Oxidation of ammonia and methane in an alka-
line, saline lake. Limnol. Oceanograph. 44, 178–188 (1999).
KALYUZHNAYA, M. G., KHMELENINA, V. N., SUZINA, N. E., LY-
T
SENKO, A. M., TROTSENKO, Y.A.: New methanotrophic isolates
from soda lakes of the southeastern Transbaikal region.
Mikrobiologiya. (English translation). 68, 677–685 (1999).
HMELENINA, V.N., KALYUZHNAYA, M. G., STAROSTINA, N. G. SU-
UZINA, N.E., TROTSENKO, Y.A.: Isolation and characterization
of halotolerant alkaliphilic methanotrophic bacteria from Tuva
soda lakes. Curr Microbiol 35, 1–5 (1997).
K
K
K
T
T
T
HMELENINA, V. N., KALYUZHNAYA, M. G., SAKHAROVSKY, V .G.
SUZINA, N. E., TROTSENKO, Y. A., GOTTSCHALK, G.: Osmoad-
aptation in halophilic and alkaliphilic methanotrophs. Arch.
Microbiol. 172, 321–329 (1999).
HMELENINA, V. N., ESHINIMAEV B. T
S., KALYUZHNAYA, M. G.,
T
ROTSENKO Y. A.: Potential Activity of methane and ammoni-
W
um oxidation by methanotrophic communities from the soda
lakes of Southern Transbaikal. Mikrobiologiya. (English trans-
lation). 69, 460–465 (2000a).
Z
KHMELENINA, V. N., SAKHAROVSKY, V. G., RESHETNIKOV, A. S.,
T
ROTSENKO, Y. A.: Synthesis of osmoprotectants by halophilic
and alkaliphilic methanotrophs. Mikrobiologiya. (English
translation). 69, 381–386 (2000b).
Corresponding author:
L
ANG, E., LANG, H.: Spezifische Farbreaktion zum direkten Nach-
weise der Ameisensaure. Z. Anal. Chem. 260, 8–10 (1960).
IPPERT, K., GALINSKI, E. A.: Enzyme stabilization by ectoine-type
compatible solutes: protection against heating, freezing and
drying. Appl. Microbiol. Biotech. 37, 61–65 (1992).
YURI A. TROTSENKO, G. K. SKRYABIN Institute of Biochemistry
and Physiology of Microorganisms RAS, Pushchino, Moscow
region, 142290, Russia
Tel: ++7-095-925 7478; Fax: ++7-095-956 3370;
e-mail: trotsenko@ibpm.serpukhov.su
L