Microwaves and Aqueous Solvents Promote the Reaction of Poorly Nucleophilic Anilines with a Zincke Salt

Article abstract of DOI:10.1021/acs.joc.6b00208

The Zincke reaction allows the transformation of primary amines into their respective N-alkylated or N-arylated pyridinium salts. While nucleophilic primary amines (typically, aliphatic primary amines) often lead to quantitative reactions and has been doc

Full text of DOI:10.1021/acs.joc.6b00208

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Article
Microwaves and aqueous solvents promote the reaction
of poorly nucleophilic anilines with a Zincke salt.
Narimane Zeghbib, Paul Thelliere, Michael Rivard, and Thierry Martens
J. Org. Chem., Just Accepted Manuscript • DOI: 10.1021/acs.joc.6b00208 • Publication Date (Web): 17 Mar 2016
Downloaded from http://pubs.acs.org on March 18, 2016
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Microwaves and Aqueous Solvents Promote the Reaction of Poorly
Nucleophilic Anilines with a Zincke Salt
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*
Narimane Zeghbib, Paul Thelliere, Michael Rivard and Thierry Martens
Université Paris Est, ICMPE (UMR 7182), CNRS, UPEC, 94320 Thiais, France
*Corresponding author: rivard@icmpe.cnrs.fr
Abstract graphic
Abstract
The Zincke reaction allows the transformation of primary amines into their respective N-alkylated or N-arylated
pyridinium salts. While nucleophilic primary amines (typically, aliphatic primary amines) often lead to quantitative
reactions and has been documented profusely, the use of poorly nucleophilic amines still requires an in depth account.
To date, the lack of nucleophilicity of the amines is redhibitory. The subject addressed in this article is a series of
primary amines deriving from aniline having been engaged in Zincke reactions. Efficient transformations were
obtained, even when conducted on electronically deactivated, eventually also sterically hindered, substrates. This was
achieved by the combined use of microwave activation and aqueous solvents. Under our conditions, the role of water
revealed indeed crucial to avoid the self-degradation of the Zincke salt, the reagent of the reaction.
Introduction
The Zincke reaction allows the transformation of primary amines 1 into their respective N-alkyl- or N-aryl-
pyridinium salts 2 (scheme 1).1,2 This reaction usually requires N-(2,4-dinitrophenyl)-pyridinium chloride 3 as
reagent, the latter being commonly called Zincke salt.3 Following a SN(ANRORC) mechanism (i.e. a Nucleophilic
Substitution by Adding the Nucleophile, Ring Opening, and Ring Closure),4-6 the Zincke reaction consists of a
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transannulation reaction by transferring the Zincke salt pyridinium ring carbon chain onto the nitrogen atom of the
primary amine. Concomitantly, 2,4-dinitroaniline 4 is released as by-product. The Zincke reaction is typically
performed under thermal activation but also proceeds under unconventional activation methods such as microwaves
or ultrasonic activation.7-10 The ease with which this reaction is carried out makes it the reaction of choice when
preparing N-substituted pyridinium salts either in solution or on solid-phase.11,12 The extensive applicability of
pyridinium salts, prepared per se or as intermediates in heterocyclic chemistry, explains the abundant literature
mentioning the use of the Zincke reaction.13
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Scheme 1. The Zincke reaction
The Zincke reaction commonly results in good to excellent yields, especially when using aliphatic primary amines.
However, an overview of the literature allows identifying conditions leading to moderate or even low yields. Such
situations are typically associated with the formation of macrocycles14,15 and/or the use of aniline derivatives as
primary amines.16-20
Recently, we described the preparation of an N-arylated pyridinium.21 Later, the toxicological evaluation of this
compound showed its ability to induce cell death by apoptosis on human neuroblastoma cells (SH-SY5Y).22 Based on
these preliminary results, we next aimed to prepare a series of N-arylated pyridiniums by Zincke reaction, including
challenging compounds deriving from poorly nucleophilic anilines. In order to obtain these target pyridinums in good
yields, we logically envisioned the microwave activation.23,24 Under these conditions however and when poorly
nucleophilic anilines are used as primary amines, side reactions turn into a challenge as they are also significantly
accelerated.
In the present article, we describe the efficient synthesis by the Zincke reaction of N-arylated pyridiniums with a
focus on compounds deriving from poorly nucleophilic anilines, most of which remain inaccessible under
conventional activation methods and consequently constitute a sparsely-described class of compounds. We also
demonstrate the crucial role of water used as co-solvent in order to achieve their preparation.
Results and discussion
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Stability study on the Zincke salt 3
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As the microwave heating allows reaching very high temperatures, a stability study was conducted on the Zincke salt
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3 (scheme 1). For this, the salt was dissolved in different solvents and then heated under microwave irradiation and
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under pressure at 130 and 150 °C. The degradation of the salt 3 was measured by H NMR in DMSO-d6, in the
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presence of dichloroethane used as external standard (Table 1).
Table 1. Stability of the Zincke salt 3 in various solvents
entry
solvent
Degradation of 3 [%] a
130 °C
95
150 °C
98
1
2
3
4
MeCN
i-PrOH
EtOH
H2O
50
97
20
76
0
0
conditions : MW activation under pressure, 5 min, Zincke
salt 3 (0.2 mmol), solvent (1 mL). a: measured by 1H
NMR in DMSO-d6 in the presence of dichloroethane as
external standard.
The stability study first showed that the Zincke salt 3 disappeared almost completely after 5 minutes of heating in
MeCN at 130 and 150 °C (entry 1). The presence of chlorodinitrobenzene and pyridine highlighted by 1H NMR led to
conclude unambiguously that a SNAr reaction occurred by ipso substitution of the pyridinium ring by chloride ions –
which is in fact the reverse reaction of the formation reaction of the Zincke salt. It should be noted that this reaction
of self-degradation, consecutive to the nucleophilic attack of chloride ions, has already been reported. The solution
proposed to avoid this self-degradation was a replacement by salt metathesis of chloride ions by non-nucleophilic
alkyl sulfates.11,25
The stability study also showed that protic solvents such as alcohols were able to minimize this reaction of self-
degradation due to their ability to solvate chloride ions.26 As expected, the more the solvation was effective, the more
the self-degradation was limited. Thus EtOH, more acidic than i-PrOH, revealed to be also more efficient to preserve
the Zincke salt 3 (entries 2 and 3). The increase of molecular agitation due to the raising of the temperature, by
minimizing the solvation effect, also resulted in a significant increase of the self-degradation. It should be noted that
the solvents commonly used for the Zincke reaction are C1-C5 alcohols, typically EtOH or n-BuOH. The fact that
excellent yields can be obtained with this type of solvent suggests that in the presence of sufficiently nucleophilic
primary amines (typically, aliphatic amines) this possibly competitive self-degradation reaction – in reality – remains
limited, even under microwave activation.7
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Considering the use of water as solvent, it was found that in this medium, the Zincke salt 3 remained intact after 5
minutes of heating at 130 and even 150 °C (entry 4). It should be noted that the use of water as solvent for the Zincke
reaction has already been described, including under microwave irradiation. However, its protective effect by
solvation of chloride ions has, to our knowledge, never been demonstrated nor even mentioned.8,27 The lipophilicity
of some primary amines unfortunately made it impossible to use only water as the reaction solvent. Considering
logically using a EtOH/H2O binary solvent, we endeavoured to determine the optimal proportion of these two co-
solvents for an effective implementation of the Zincke reaction.
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Optimization of the binary solvent
The optimization of the EtOH/H2O binary solvent was performed under microwave irradiation at 150 °C and under
pressure on a reaction between the Zincke salt 3 and p-toluidine 1a, the latter being chosen precisely for its
lipophilicity (Figure 1). The yields in the expected salt 2a and of the self-degradation of the Zincke salt 3 were
1
measured after 5 minutes of reaction by H NMR in DMSO-d6, in the presence of diethyl fumarate used as external
standard.
Figure 1. Determination of the optimal composition of the EtOH/H2O binary solvent
yield of 2a [%]
degradaꢀon of 3 [%]
100
80
60
40
20
0
0
20
40 60
EtOH [%]
80
100
Conditions : MW activation (150 °C, under pressure), 5 min, Zincke salt 3 (0.2 mmol),
1a (2 eq), solvent (1 mL). Yields and degradation were measured by 1H NMR in
DMSO-d6 in the presence of diethyl fumarate as external standard.
This study showed that the use of water as single reaction solvent preserved the Zincke salt 3, merely gave a limited
yield of 32% of the expected salt 2a. The lack of solubility of the aniline 1a in the reaction mixture presumably
explains this result. The addition of ethanol helped to significantly improve the yields while limiting the self-
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degradation of the Zincke salt 3. From this study, it appeared that a EtOH/H2O mixture, with volume ratio in the
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range of (60/40) to (80/20), was the optimal solvent to carry out Zincke reactions.
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Reactivity study on the aniline derivative used as primary amine
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As second partner of the reaction, the aniline derivative used as primary amine was also subjected to a preliminary
study. More precisely, it appeared useful to identify the parameters relevant to anticipate its reactivity with the Zincke
salt 3. For this, a series of variously functionalized monosubstituted anilines was engaged in a Zincke reaction, under
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conventional heating at 80 °C, for 96 hours, in an EtOH/H2O (60/40) binary solvent. Yields were measured by H
NMR in DMSO-d6, in the presence of diethyl fumarate used as external standard.
This study showed a good correlation between the yield of the reaction and the pKa of the conjugate acid of the
considered aniline derivative (Figure 2).28-31 In our conditions, compounds associated to pKa superior to 3.75 gave the
corresponding salt in quantitative yields. Conversely, aniline derivatives associated to pKa inferior to 2.75,
characterized by the electronic deactivation of the nitrogen atom, eventually associated to a steric hindrance, gave the
expected salts in low yields of about 20% maximum. It should be noted that when no or incomplete conversions were
observed, the amount of starting materials was measured by NMR. The measured quantities were consistent with
those expected, thus confirming the absence of any competitive self-degradation reaction.
Figure 2. Reactivity study on aniline derivatives
p-OH
p-Cl o-Me m-Me
m-Cl
100
80
60
40
20
0
H
p-Me
m-CF3
m-COOH
m-CN
p-COOH o-Cl
o-CF3
p-CN
o-CN
0.5
1
1.5
2
2.5
3
3.5
4
4.5
5
5.5
pKa(b)
Conditions : conventionnal heating (80 °C), 96 h, Zincke salt 3 (0.2 mmol),
RC6H4NH2 (2 eq), EtOH/H2O : 60/40 (1 mL). a: yields were measured by 1H NMR in
DMSO-d6 in the presence of diethyl fumarate as external standard. b: for pKa values,
see ref 28-31.
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It should be noted that a reaction was carried out in the presence of a larger excess of non-reactive aniline. The
Zincke salt 3 (0.1M) was thus engaged with 6 equivalents of o-trifluoromethyl aniline 1b. After 18 weeks of reaction
under conventional heating in refluxing ethanol, an inseparable (and thus unusable) mixture of Zincke salt and
expected salt 2b was obtained. The incomplete conversion of about 78% measured by NMR confirmed the need to
develop a protocol both applicable to poorly nucleophilic primary amines and reasonably feasible from a practical
viewpoint.
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Implementation of the Zincke reaction with aniline derivatives
A series of aniline derivatives 1 was put to react with the Zincke salt 3, under microwave irradiation and under
pressure in an EtOH/H2O (60/40) binary solvent. Three different protocols were set up depending on the pKa of the
1
primary amine used. The monitoring of the reactions was carried out by H NMR in DMSO-d6, in the presence of
diethyl fumarate used as external standard. To avoid obtaining mixtures of salts, reaction times were adjusted so that
the yields in expected salts 2 were quantitative (Table 2).
Table 2. Quantitative Zincke reactions with aniline derivatives 1a-v
entry
1
pKa [ref]
0.95 [28]
1.70 [31]
2.19 [28]
2.32 [29]
2.39 [30]
2.62 [29]
2.80 [31]
3.05 [29]
3.32 [29]
3.49 [29]
nd
R
aniline
1c
protocol
time [min]
isolated yield [%]
o-CN
A
A
A
B
A
A
B
B
B
B
B
B
C
60
60
60
60
60
75
60
60
40
60
60
20
30
84
88
2
p-CN
1d
1e
3
p-COMe
p-COOH
o-CF3
84
4b
5c
6
1f
38[90]f
8[16]f
84
1b
1g
o-Cl
7
m-CN
1h
1i
100
78
8
m-COOH
m-Cl
9
1j
91
10
11
12
13
m-CF3
p-CONH2
m-CONH2
p-Cl
1k
1l
73
80
nd
1m
1n
64
3.81 [29]
93
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14
15d
16
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20e
21
22
3.92 [29]
4.17 [29]
4.38 [29]
4.49 [29]
4.62 [29]
4.67 [29]
4.72 [29]
5.07 [29]
5.50 [29]
1-naphtyl
m-OH
o-Me
o-OMe
H
1o
1p
1q
1r
1s
B
C
C
C
C
C
C
C
C
20
40
15
30
20
20
30
15
20
70
63[84]f
94
80
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100
95
m-Me
o-OH
p-Me
p-OH
1t
1u
1a
1v
31[77]f
96
63
Conditions: MW activation, under pressure, Zincke salt 3 (0.2 mmol), EtOH/H2O:60/40 (1 mL).
Protocol A : 150 °C, 1 (12 eq). Protocol B : 150 °C, 1 (3 eq). Protocol C : 130 °C, 1 (1.2-2 eq). a:
1
chemical yields were measured by H NMR in DMSO-d6 in the presence of diethyl fumarate as
external standard. b: the salt 2f was obtained in 42% chemical yield in mixture with side products. c:
reaction conducted in a 80/20 mixture of EtOH/H2O, the salt 2b was obtained in 50% chemical yield
(a partial decomposition of the Zincke salt 3 into chlorodinitrobenzene was observed by NMR). d: the
salt 2p was obtained in 85% chemical yield in mixture with side products. e: the salt 2u was obtained
in 40% chemical yield in mixture with side products. f: corrected yield, taking into account the
chemical yield inferior to 100%.
Reactions involving aniline derivatives whose pKa of conjugate acids is inferior to 2.75 required experimental
conditions defined in protocol A, with the noticeable exception however of the p-aminobenzoic acid 1f (entry 4).
Leading quantitatively to the expected salts, protocol A was characterized by the use of a large excess of amine (12
equivalents vs. Zincke salt 3) and prolonged heating times (60-75 min) at 150 °C (entries 1, 2, 3, 5, and 6). The large
excess of amine was necessary in order to complete the reaction. It is possible that dinitroaniline 4, released during
the reaction and the concentration of which therefore increases over time, competes with poorly nucleophilic aniline
derivatives. The competing nucleofugalities of the dinitroaniline and of the amine to be quaternized may result in the
establishment of an equilibrium in solution, which only a large excess of amine is able to completely displace. In this
series, 4’-aminoacetophenone 1e which has revealed to be totally unreactive under conventional activation,24 was
successfully converted into its corresponding salt (entry 3). It should be also noted that because of its lipophilicity, o-
trifluoromethyl aniline 1b revealed to be slightly soluble in the EtOH/H2O (60/40) binary solvent. For this reason, a
(80/20) mixture enriched in ethanol was used. This resulted in a partial self-degradation of the Zincke salt 3 into
chlorodinitrobenzene and the formation of salt 2b in 50% yield before purification (entry 5).
In the case of reactions involving this kind of poorly nucleophilic aniline derivatives, the SNAr between pyridine and
the corresponding chlorinated derivatives could be considered for the preparation of pyridinium salts. For this
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purpose, reactions were conducted under microwaves and under pressure at 150 °C in an EtOH/H2O (60/40) binary
solvent (Scheme 2).
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Scheme 2. Possible access to pyridinium chlorides by SNAr
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Despite the drastic conditions (150 °C in the presence of 10 eq of pyridine), no traces of the desired salts could be
observed after 30 min of reaction. Consequently, the use of the SNAr reaction as an alternative to the Zincke reaction
revealed to be totally ineffective, even in the case of these electronically deactivated substrates.
Reactions involving aniline derivatives 1h-k, whose pKa of conjugate acids is between 2.75 and 3.75, required the
use of only 3 equivalents of amine at 150 °C (these conditions characterizing protocol B) to give quantitatively the
corresponding salts 2h-k (entries 7-10). The salt deriving from the p-aminobenzoic acid 1f was obtained by observing
these experimental conditions. According to protocol B, anilines derivatives 1l and 1m, bearing an aminocarbonyl
substituent (pKa unknown), gave the corresponding salts 2l-m (entries 11-12).
Aniline derivatives whose pKa of conjugate acids is superior to 3.75, were quantitatively converted into their
corresponding salts following protocol C, characterized by the use of a slight excess of amine (from 1.2 to 2
equivalents), the reaction temperature lowered to 130 °C and reaction time limited to 40 minutes (entries 13, 15-22).
It should be noted however that despite a pKa of 3.92, naphthylamine 1o quantitatively gave the corresponding salt 2o
following the protocol B set up for less reactive aniline derivatives 1h-m (entry 14). This is in accordance with a
previous study showing the total lack of reactivity of 1o when engaged in a Zincke reaction under ultrasonic
activation.10 This behaviour, presumably explained by the steric hindrance of the naphtyl group, illustrates the limits
of considering only pKa values to anticipate the reactivity of primary amines engaged in the Zincke reaction. It should
also be noted that in the case of aminophenols 1p and 1u side reactions occurred, giving the expected salts in 85%
and 40% respectively (entries 15 and 20). In this series, aminophenols revealed a variable reactivity, depending on
their substitution pattern. Engaged in reaction with the Zincke salt 3, o-, m- and p-aminophenols 1u, 1v and 1p gave
indeed the expected salts in 40, 85 and 100% yield respectively (entries 20, 15 and 22). The purification of the crude
mixture obtained from the o-aminophenol 1u, allowed isolating a major side-product, fully characterized after
recrystallization in i-PrOH, and unambiguously attributed to be the diarylamine 5 (Figure 3).
Figure 3. Identification of a side-product of the Zincke reaction
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Resulting from a SNAr reaction, the diarylamine 5 may be formed as a consequence of the direct nucleophilic attack
of the amino group of the aminophenol 1u. Alternatively, it may also resulted from a first substitution led by the
hydroxyl group of 1u,32 thus giving the intermediate diaryl ether 6. In such hypothesis, the subsequent Smiles
rearrangement of 6 into 5 demonstrated at room temperature and in the presence of water, would occur.33,34 Whatever
the mechanism involved, it seems that the possibility to form such diarylamines explains the variable yields in the
expected salt, obtained when using aminophenols as primary amine. Interestingly, the protection of the hydroxyl
group under the form of a methyl ether (as in the case of o-anisidine 1r) prevented the formation of any side-products
and gave quantitatively the expected salt 2r (entry 17).
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Due to their polarity, salts obtained by the Zincke reaction are known to be purified with difficulty.35 When reactions
are conducted on large scale however, the expected salts can be purified by recrystallization.36 Regarding compounds
2a-v, they could be separated from the dinitroaniline 4 and from the excess of amine by chromatography on reversed
phase.37 Purification yields were generally good, ranging from 63 to 100%. It should be noted that salt 2b was
isolated with an overall yield of 8% (of 16% taking into consideration the yield of only 50% measured in the crude
mixture) indicating a significant loss of material during the purification step (entry 5). Deriving from an aniline
derivative among the less basic, it is possible that this salt degraded during purification due to its electrophilicity.
Conclusion
This work allowed pointing out the limit of the Zincke reaction when conducted under conventional heating. It also
allowed identifying the kind of primary amines for which the use of more efficient activation methods was not only
useful but also necessary. In this regard, microwave activation revealed to be the ideal method of obtaining
quantitative reactions, even when conducted on substrates for which the conventional heating or even unconventional
methods, such as ultrasonic activation, remain inoperative. Under these conditions however, the use of water is
crucial in order to preserve Zincke salt 3 from self-degradation by solvation of chloride ions. With exception to the
particularly hindered substrates, as highlighted in this study, the good correlation observed between pKa values and
the reactivity of anilines derivatives should enable to choose the correct protocol for the quantitative transformation
of any primary amine of known pKa. The present study should consequently help to significantly extend the scope of
the Zincke reaction, especially regarding poorly nucleophilic primary amines.
Experimental section
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Materials and methods
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All chemicals and solvents were used as received without purification. EtOH purity was of analysis grade (water ≤
200 mg/Kg) and water was distillated. The Zincke salt 3 was prepared according to the reported procedure.38
Reactions were carried out in closed vials using a CEM-Discover apparatus, provided with a fiber optic temperature
control. Automated purification of salts 2a-v was performed on C18 reversed-phase (40 g cartridges). NMR spectra
were recorded in DMSO-d6 or MeOH-d4. Chemical shifts (δ) are reported in part per million (ppm) relative to the
residual no-deuterated solvent signal. Coupling constant values (J) are given in hertz (Hz) and refer to apparent
multiplicities indicated as follows: s (singlet); d (doublet); t (triplet); q (quadruplet); m (multiplet); dd (doublet of
doublets); td (triplet of doublets); bp (broad peak). IR spectra were recorded on a spectrometer equipped with a
diamond ATR unit, between 500 and 4000 cm-1. Wavenumbers (ν) are expressed in cm-1 at their maximum intensity.
LC-MS analyses were performed with a reversed-phase chromatographic system (MeOH was used as eluent in
isocratic mode) coupled with a single-quadrupole mass spectrometer (ESI+ mode, 50 V, 400 °C). GC-MS analysis
was performed in ionization mode (EI+). CHN analyses were done by the elemental analysis service of the “Institut
de Chimie des Substances Naturelles”, Gif-sur-Yvette, France.
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General procedure for the synthesis of pyridinium salts 2a-v
Into a microwave vial (10 mL) equipped with a magnetic stirrer, were successively added the Zincke salt 3, the
specified quantity of aniline derivative 1, and the EtOH/H2O binary solvent (1 mL). Unless specified, the reaction
was conducted on 0.2 mmol (56.2 mg) of Zincke salt 3, in a EtOH/H2O (60/40) mixture. The reaction was performed
in closed vial for the specified time, and under microwaves (150 W) at the specified temperature. After completion of
the reaction, the mixture was concentrated under vacuum. The crude product was purified by chromatography on
reverse phase, according to the specified method (vide infra). The expected salt was obtained after evaporation of the
mobile phase.
General procedure for the purification of pyridinium salts 2a-v
Method 1: impurities were eluted by successively Et2O (5 column volumes) and MeCN (5 column volumes); the
expected salt was obtained after elution with MeOH (3 column volumes). Method 2: impurities were eluted by MeCN
(5 column volumes) and the expected salt was obtained after elution with MeOH (3 column volumes). Method 3: the
expected salt was obtained in the very first fraction eluted with a H2O/MeOH (95/5) mixture.
1-(p-tolyl)pyridin-1-ium chloride (2a).36,39 2a was prepared from 1a (25.7 mg, 0.24 mmol, 1.2 eq) after 20 min of
heating under microwaves at 130 °C, and purified according to method 2. 2a was obtained under the form of a beige
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powder (39.5 mg, 96%). H NMR (400 MHz, MeOH-d4) δ 9.26 (dd, 2H, J = 7.5, 1.2 Hz), 8.80 (tt, 1H, J = 7.5, 1.2
Hz), 8.31 (t, 2H, J = 7.5 Hz), 7.75 (d, 2H, J = 8.3 Hz), 7.75 (d, 2H, J = 8.3 Hz), 2.51 (s, 3H). 13C NMR (100 MHz,
MeOH-d4) δ 147.6, 146.0, 143.6, 142.2, 132.1, 129.6, 125.2, 21.2. IR (neat) ν 3451, 3397, 3015, 1778, 1477, 1453,
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833, 787, 694. LC-MS m/z (%) 170 (100), 171 (12). Anal calcd for C12H12ClN.4/3H2O: C, 62.75; H, 6.44; N, 6.10.
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Found: C, 62.58; H, 6.19; N, 6.51.
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1-(2-(trifluoromethyl)phenyl)pyridin-1-ium chloride (2b). 2b was prepared from 1b (386.7 mg, 2.4 mmol, 12 eq)
after 60 min of heating under microwaves at 150 °C in a EtOH/H2O (80/20) mixture. 2b was purified according to
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method 1 and obtained under the form of a brown powder (4.0 mg, 8%). H NMR (400 MHz, MeOH-d4) δ 9.31 (d,
2H, J = 7.0 Hz), 8.96 (t, 1H, J = 7.0 Hz), 8.40 (t, 2H, J = 7.0 Hz), 8.12 (dd, 1H, J = 7.0, 1.7 Hz), 8.08-7.99 (bp, 3H).
13C NMR (100 MHz, MeOH-d4) δ 149.7, 148.1, 140.7, 135.8, 133.9, 129.9, 129.3, 129.1 (q, J = 4.9 Hz), 126.2 (q, J
= 32.0 Hz), 124.0 (q, J = 273.0 Hz). 19F NMR (376 MHz, MeOH-d4) δ -59.81. IR (neat) ν 3385, 3033, 1502, 1486,
1469, 1129, 1109, 1074, 793, 685. LC-MS m/z (%) 224 (100). Anal calcd for C12H9ClF3N.1/2H2O: C, 53.65; H, 3.75;
N, 5.21. Found: C, 53.27; H, 3.70; N, 5.40.
1-(2-cyanophenyl)pyridin-1-ium chloride (2c). 2c was prepared from 1c (283.5 mg, 2.4 mmol, 12 eq) after 60 min of
heating under microwaves at 150 °C, and purified according to method 1. 2c was obtained under the form of a very
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hygroscopic turquoise powder (36.5 mg, 84%). H NMR (400 MHz, MeOH-d4) δ 9.40 – 9.34 (m, 2H), 8.95 (tt, J =
7.8, 1.3 Hz, 1H), 8.42 (dd, J = 7.8, 6.8 Hz, 2H), 8.17 (dd, J = 7.6, 1.3 Hz, 1H), 8.11 – 8.01 (m, 2H), 7.97 (td, J = 7.6,
1.5 Hz, 1H). 13C NMR (100 MHz, MeOH-d4) δ 149.8, 147.3, 144.9, 136.4, 135.7, 133.6, 129.8, 128.5, 115.3, 110.6.
IR (neat) ν 3357, 3016, 2240, 1494, 1469, 1356, 779, 689. LC-MS m/z (%) 181 (100), 182 (13). Anal calcd for
C12H9ClN2.5/4H2O: C, 60.26; H, 4.85; N, 11.71. Found: C, 60.43; H, 4.85; N, 11.63.
1-(4-cyanophenyl)pyridin-1-ium chloride (2d).36 2d was prepared from 1d (283.5 mg, 2.4 mmol, 12 eq) after 60 min
of heating under microwaves at 150 °C, and purified according to method 1. 2d was obtained under the form of a
white powder (38.1 mg, 88%). 1H NMR (400 MHz, DMSO-d6) δ 9.38 (d, 2H, J = 7.6 Hz), 8.83 (t, 1H, J = 7.6 Hz),
8.35 (t, 2H, J = 7.6 Hz), 8.30 (d, 2H, J = 8.7 Hz), 8.12 (d, 2H, J = 8.7 Hz). 13C NMR (100 MHz, DMSO-d6) δ 147.4,
145.6, 145.1, 134.3, 128.1, 126.3, 117.6, 114.1. IR (neat) ν 3366, 3031, 3006, 2235, 1486, 1471, 784, 678. LC-MS
m/z (%) 181 (100), 182 (14), 183 (1), 246 (17), 247 (2). Anal calcd for C12H9ClN2.5/4H2O: C, 60.26; H, 4.85; N,
11.71. Found: C, 60.70; H, 4.96; N, 11.25.
1-(4-acetylphenyl)pyridin-1-ium chloride (2e). 2e was prepared from 1e (324 mg, 2.4 mmol, 12 eq) after 60 min of
heating under microwaves at 150 °C, and purified according to method 2. 2e was obtained under the form of a brown
powder (40.3 mg, 84%). 1H NMR (400 MHz, MeOH-d4) δ 9.37 (d, 2H, J = 7.1 Hz), 8.88 (t, 1H, J = 7.1 Hz), 8.38 (t,
2H, J = 7.1 Hz), 8.34 (d, 2H, J = 7.9 Hz), 8.05 (d, 2H, J = 7.9 Hz), 2.73 (s, 3H). 13C NMR (100 MHz, MeOH-d4) δ
198.7, 148.5, 147.2, 146.0, 140.5, 131.6, 129.7, 126.2, 27.1. IR (neat) ν 3334, 3031, 1674, 1597, 1475, 1362, 1257,
786. LC-MS m/z (%) 198 (100), 199 (19). Anal calcd for C13H12ClNO.7/5H2O: C, 60.31; H, 5.76; N, 5.41. Found: C,
60.35; H, 6.04; N, 5.25.
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1-(4-carboxyphenyl)pyridin-1-ium chloride (2f). 2f was prepared from 1f (82.3 mg, 0.6 mmol, 3 eq) after 60 min of
heating under microwaves at 150 °C, and purified according to method 1. 2f was obtained under the form of a brown
solid (18.1 mg, 38%). 1H NMR (400 MHz, MeOH-d4) δ 9.32 (d, 2H, J = 7.8 Hz), 8.84 (t, 1H, J = 7.8 Hz), 8.34 (bp,
4H), 7.96 (d, 2H, J = 8.5 Hz). 13C NMR (100 MHz, MeOH-d4) δ 168.1, 148.4, 147.3, 146.1, 135.5, 132.8, 129.6,
125.9. IR (neat) ν 3382, 3015, 2776, 2584, 1699, 1624, 1473, 1385, 1328, 1236, 791, 682. LC-MS m/z (%) 200 (100),
202 (13). Anal calcd for C12H10ClNO2.H2O: C, 56.81; H, 4.77; N, 5.52. Found: C, 56.83; H, 4.64; N, 5.49.
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1-(2-chlorophenyl)pyridin-1-ium chloride (2g).40 2g was prepared from 1g (306.2 mg, 2.4 mmol, 12 eq) after 75 min
of heating under microwaves at 150 °C, and purified according to method 1. 2g was obtained under the form of a very
hygroscopic green solid (38.0 mg, 84%). 1H NMR (400 MHz, MeOH-d4) δ 9.22 (d, 2H, J = 7.1 Hz), 8.92 (t, 1H, J =
7.1 Hz), 8.39 (t, 2H, J = 7.1 Hz), 7.91 (d, 1H, J = 7.8 Hz), 7.85 (d, 1H, J = 7.8 Hz), 7.79 (t, 1H, J = 7.8 Hz), 7.72 (t,
1H, J = 7.8 Hz). 13C NMR (100 MHz, MeOH-d4) δ 149.3, 147.2, 141.3, 134.5, 132.3, 130.3, 129.8, 129.7, 128.9. IR
(neat) ν 3363, 3024, 1470, 762, 681. LC-MS m/z (%) 190 (100), 191 (16), 192 (49). Anal calcd for C11H9Cl2N.H2O:
C, 54.12; H, 4.54; N, 5.74. Found: C, 53.80; H, 4.48; N, 5.57.
1-(3-cyanophenyl)pyridin-1-ium chloride (2h). 2h was prepared from 1h (70.8 mg, 0.6 mmol, 3 eq) after 60 min of
heating under microwaves at 150 °C, and purified according to method 2. 2h was obtained under the form of a brown
powder (43.3 mg, 100%). 1H NMR (400 MHz, MeOH-d4) δ 9.37 (d, 2H, J = 7.0 Hz), 8.90 (t, 1H, J = 7.0 Hz), 8.40
(s, 1H), 8.37 (d, 2H, J = 7.0 Hz), 8.26 (d, 1H, J = 8.1 Hz), 8.17 (d, 1H, J = 8.1 Hz), 7.99 (t, 1H, J = 8.1 Hz). 13C NMR
(100 MHz, MeOH-d4) δ 148.7, 146.3, 144.6, 136.3, 132.9, 130.6, 129.8, 129.7, 118.0, 115.4. IR (neat) ν 3389, 3015,
2234, 1490, 1468, 776, 681. LC-MS m/z (%) 181 (100), 182 (14). Anal calcd for C12H9ClN2.7/8H2O: C, 62.01; H,
4.66; N, 12.05. Found: C, 62.00; H, 4.39; N, 12.27.
1-(3-carboxyphenyl)pyridin-1-ium chloride (2i). 2i was prepared from 1i (82.2 mg, 0.6 mmol, 3 eq) after 60 min of
heating under microwaves at 150 °C, and purified according to method 2. 2i was obtained under the form of a purple
solid (36.7 mg, 78%). 1H NMR (400 MHz, MeOH-d4) δ 9.27 (dd, 2H, J = 6.9, 1.3 Hz), 8.78 (tt, 1H, J = 7.8, 1.3 Hz),
8.28 (bp, 3H), 8.24 (m, 1H), 7.85 (ddd, 1H, J = 7.8, 2.3, 1.1 Hz), 7.74 (t, 1H, J = 7.8 Hz). 13C NMR (100 MHz,
MeOH-d4) δ 172.0, 147.8, 146.2, 144.1, 142.2, 133.2, 131.3, 129.5, 126.8, 126.0. IR (neat) ν 3378, 3041, 2688, 2535,
1704, 1625, 1469, 1443, 1378, 1290, 1257, 756, 682. LC-MS m/z (%) 200 (100), 201 (13). Anal calcd for
C12H10ClNO2.H2O: C, 56.81; H, 4.77; N, 5.52. Found: C, 56.78; H, 4.69; N, 5.53.
1-(3-chlorophenyl) pyridin-1-ium chloride (2j).39 2j was prepared from 1j (76.5 mg, 0.6 mmol, 3 eq) after 40 min of
heating under microwaves at 150 °C, and purified according to method 2. 2j was obtained under the form of a brown
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solid (41.1 mg, 91%). H NMR (400 MHz, DMSO) δ 9.39 (d, J = 5.6 Hz, 2H), 8.82 (t, J = 7.8 Hz, 1H), 8.37 – 8.27
(m, 2H), 8.15 (t, J = 2.0 Hz, 1H), 7.92 – 7.75 (m, 3H).13C NMR (100 MHz, DMSO-d6) δ 147.1, 145.1, 143.6, 134.1,
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131.8, 131.2, 128.0, 125.3, 123.9. IR (neat) ν 3360, 3071, 3025, 1627, 1588, 1471, 1093, 874, 780. LC-MS m/z (%)
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190 (100), 192 (29). Anal calcd for C11H9Cl2N.8/7H2O: C, 53.56; H, 4.61; N, 5.68. Found: C, 53.05; H, 4.49; N, 6.17.
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1-(3-(trifluoromethyl)phenyl)pyridin-1-ium chloride (2k). 2k was prepared from 1k (96.7 mg, 0.6 mmol, 3 eq) after
60 min of heating under microwaves at 150 °C, and purified according to method 1. 2k was obtained under the form
of a brown powder (37.9 mg, 73%).1H NMR (400 MHz, MeOH-d4) δ 9.32 (dd, J = 6.8, 1.3 Hz, 2H), 8.84 (tt, J = 7.8,
1.3 Hz, 1H), 8.35 – 8.30 (m, 2H), 8.28 (s, 1H), 8.12 (t, J = 7.3 Hz, 2H), 7.98 (t, J = 8.0 Hz, 1H). 13C NMR (100 MHz,
MeOH-d4) δ 148.8, 146.4, 144.7, 133.5 (q, J = 33.6 Hz), 132.9, 130.1, 129.9, 129.7, 129.4 (q, J = 3.6 Hz), 124.6 (q, J
= 221.1 Hz), 123.2 (q, J = 3.9 Hz). 19F NMR (376 MHz, MeOH-d4) δ -63.87. IR (neat) ν 3301, 3003, 1502, 1474,
1326, 1176, 1102, 775, 680. LC-MS m/z (%) 224 (100), 225 (13). Anal calcd for C12H9ClF3N.3/2H2O: C, 50.28; H,
4.22; N, 4.89. Found: C, 50.35; H, 4.25; N, 5.22.
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1-(4-carbamoylphenyl)pyridin-1-ium chloride (2l). 2l was prepared from 1l (81.6 mg, 0.6 mmol, 3 eq) after 60 min
of heating under microwaves at 150 °C, and purified according to method 2. 2l was obtained under the form of a
brown powder (40.0 mg, 85%). 1H NMR (400 MHz, MeOH-d4) δ 9.32 (d, 2H, J = 6.9 Hz), 8.84 (t, 1H, J = 6.9 Hz),
8.33 (t, 2H, J = 6.9 Hz), 8.23 (d, 2H, J = 8.6 Hz), 7.97 (d, 2H, J = 8.6 Hz). 13C NMR (100 MHz, MeOH-d4) δ 170.0,
148.4, 146.5, 146.1, 138.2, 131.0, 129.7, 126.0. IR (neat) ν 3392, 3034, 1683, 1466, 1389, 711, 672. LC-MS m/z (%)
199 (100), 200 (13). Anal calcd for C12H11ClN2O.1/2H2O: C, 59.14; H, 4.96; N, 11.50. Found: C, 59.06; H, 4.50; N,
11.59.
1-(3-carbamoylphenyl)pyridin-1-ium chloride (2m). 2m was prepared from 1m (81.6 mg, 0.6 mmol, 3 eq) after 20
min of heating under microwaves at 150 °C, and purified according to method 2. 2m was obtained under the form of
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a brown powder (32.2 mg, 68%). H NMR (400 MHz, MeOH-d4) δ 9.31 (dd, J = 6.8, 1.3 Hz, 2H), 8.83 (tt, J = 7.9,
1.3 Hz, 1H), 8.36 – 8.28 (m, 3H), 8.27 – 8.21 (m, 1H), 8.02 (ddd, J = 8.0, 2.4, 0.9 Hz, 1H), 7.86 (t, J = 8.0 Hz, 1H).
13C NMR (100 MHz, MeOH-d4) δ 169.6, 148.3, 146.2, 144.4, 137.7, 132.0, 131.6, 129.7, 128.6, 125.1. IR (neat) ν
3471, 3118, 2923, 1661, 1499, 1487, 764, 673. LC-MS m/z (%) 199 (100), 200 (14). Anal calcd for
C12H11ClN2O.2H2O: C, 53.24; H, 5.58; N, 10.35. Found: C, 53.42; H, 5.24; N, 10.49.
1-(4-chlorophenyl)pyridin-1-ium chloride (2n).36,39 2n was prepared from 1n (51.0 mg, 0.4 mmol, 2 eq) after 30 min
of heating under microwaves at 130 °C, and purified according to method 1. 2n was obtained under the form of a
light brown powder (41.9 mg, 93%). 1H NMR (400 MHz, DMSO-d6) δ 9.34 (d, 2H, J = 7.5 Hz), 8.79 (t, 1H, J = 7.5
Hz), 8.31 (t, 2H, J = 7.5 Hz), 7.94 (d, 2H, J = 8.9 Hz), 7.86 (d, 2H, J = 8.9 Hz). 13C NMR (100 MHz, DMSO-d6) δ
146.9, 145.1, 141.6, 136.1, 130.1, 128.1, 126.8. IR (neat) ν 3626, 3363, 3093, 3020, 1625, 1590, 1474, 1093, 870,
843, 777. LC-MS m/z (%) 190 (100), 191 (13), 192 (26), 415 (4), 418 (7). Anal calcd for C11H9Cl2N.H2O: C, 54.12;
H, 4.54; N, 5.74. Found: C, 54.55; H, 4.58; N, 5.72.
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1-(naphthalen-1-yl) pyridin-1-ium chloride (2o).40 2o was prepared from 1o (85.9 mg, 0.6 mmol, 3 eq) after 20 min
of heating under microwaves at 150 °C, and purified according to method 1. 2o was obtained under the form of a very
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hygroscopic red solid (33.7 mg, 70%). H NMR (400 MHz, MeOH-d4) δ 9.29 – 9.23 (m, 2H), 8.93 (tt, J = 7.9, 1.4
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Hz, 1H), 8.39 (dd, J = 7.8, 6.7 Hz, 2H), 8.32 (d, J = 8.4 Hz, 1H), 8.21 – 8.15 (m, 1H), 7.92 (dd, J = 7.4, 1.1 Hz, 1H),
7.82 – 7.67 (m, 3H), 7.39 – 7.35 (m, 1H). 13C NMR (100 MHz, MeOH-d4) δ 148.8, 147.9, 140.4, 135.6, 133.4, 130.4,
129.9, 129.8, 129.1, 128.4, 126.5, 125.5, 121.3. IR (neat) ν 3364, 3034, 1509, 1469, 774, 684. LC-MS m/z (%) 206
(100), 207 (15). Anal calcd for C15H12ClN.H2O: C, 69.36; H, 5.43; N, 5.39. Found: C, 69.49; H, 5.38; N, 5.41.
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1-(3-hydroxyphenyl)pyridin-1-ium chloride (2p). 2p was prepared from 1p (43.6 mg, 0.4 mmol, 2 eq) after 40 min
of heating under microwaves at 130 °C, and purified according to method 2. 2p was obtained under the form of a
very hygroscopic black solid (26.4 mg, 63%). 1H NMR (400 MHz, MeOH-d4) δ 9.22 (d, 2H, J = 6.5Hz), 8.78 (t, 1H,
J = 6.5 Hz), 8.27 (t, 2H, J = 6.5 Hz), 7.54 (t, 1H, J = 9.8 Hz), 7.24 (d, 1H, J = 9.8 Hz), 7.22 (s, 1H), 7.15 (d, 1H, J =
9.8 Hz). 13C NMR (100 MHz, MeOH-d4) δ 160.6, 147.9, 146.0, 145.5, 132.6, 129.5, 119.6, 115.9, 112.4. IR (neat) ν
= 3485, 3047, 3026, 1567, 1480, 1335, 1297, 1252, 805, 794. LC-MS m/z (%) 172 (100), 173 (13), 343 (17).
Anal calcd for C11H10ClNO.2H2O: C, 54.22; H, 5.79; N, 5.75. Found: C, 54.59; H, 5.88; N, 5.59.
1-(o-tolyl)pyridin-1-ium chloride (2q).40 2q was prepared from 1q (42.8 mg, 0.4 mmol, 2 eq) after 15 min of heating
under microwaves at 130 °C, and purified according to method 2. 2q was obtained under the form of a brown powder
(38.9 mg, 94%). 1H NMR (400 MHz, MeOH-d4) δ 9.13 (dd, 2H, J = 6.7, 1.3 Hz), 8.86 (tt, 1H, J = 6.7, 1.3 Hz), 8.34
(t, 2H, J = 6.7 Hz, 7.68-7.53 (bp, 4H), 2.21 (s, 3H). 13C NMR (100 MHz, MeOH-d4) δ 148.3, 147.3, 143.8, 133.9,
133.2, 132.9, 129.7, 129.0, 126.9, 17.0. IR (neat) ν 3365, 3118, 2923, 1487, 1469, 1385, 767, 717, 685. LC-MS m/z
(%) 170 (100), 171 (13). Anal calcd for C12H12ClN.7/4H2O: C, 60.76; H, 6.59; N, 5.90. Found: C, 60.93; H, 6.28; N,
5.99.
1-(2-methoxyphenyl)pyridin-1-ium chloride (2r).40 2r was prepared from 1r (49.3 mg, 0.4 mmol, 2 eq) after 30 min
of heating under microwaves at 130 °C, and purified according to method 2. 2r was obtained under the form of a light
brown powder (35.5 mg, 80%). 1H NMR (400 MHz, DMSO-d6) δ 9.24 (dd, 2H, J = 6.5, 1.2 Hz), 8.81 (tt, 1H, J = 6.5,
1.2 Hz), 8.32 (t, 2H, J = 6.5 Hz), 7.77 (d, 1H, J = 7.9 Hz), 7.72 (t, 1H, J = 7.9 Hz), 7.46 (d, 1H, J = 7.9 Hz), 7.28 (t,
1H, J = 7.9 Hz), 3.86 (s, 3H). 13C NMR (100 MHz, DMSO-d6) δ 151.8, 147.1, 146.7, 133.0, 131.2, 127.9, 126.9,
121.2, 113.4, 56.6. IR (neat) ν 3440, 3380, 3031, 2841, 1606, 1440, 1277, 1020, 763, 684, 694. LC-MS m/z (%) 186
(100), 187 (17). Anal calcd for C12H12ClNO.4/3H2O: C, 58.66; H, 6.02; N, 5.70. Found: C, 58.82; H, 5.66; N, 5.86.
1-phenylpyridin-1-ium chloride (2s).36 2s was prepared from 1s (22.3 mg, 0.24 mmol, 1.2 eq) after 20 min of heating
under microwaves at 130 °C, and purified according to method 2. 2s was obtained under the form of a brown powder
(38.3 mg, 100%). 1H NMR (400 MHz, DMSO-d6) δ 9.37 (d, 2H,J = 7.1 Hz), 8.79 (t, 1H, J = 7.1 Hz), 8.32 (t, 2H, J =
7.1 Hz), 7.90 (dd, 2H, J = 5.9, 1.9 Hz), 7.75 (bp, 3H). 13C NMR (100 MHz, DMSO-d6) δ 146.6, 145.0, 142.8, 131.2,
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130.2, 128.2, 124.8. IR (neat) ν 3626, 3363, 3093, 3020, 1625, 1590, 1474, 870, 843, 777. LC-MS m/z (%) 190 (100),
191 (13), 192 (26), 415 (4), 418 (7). Anal calcd for C11H10ClN.3/2H2O: C, 60.42; H, 5.99; N, 6.41. Found: C, 60.35;
H, 5.68; N, 6.14.
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1-(m-tolyl)pyridin-1-ium chloride (2t).36 2t was prepared from 1t (25.7 mg, 0.24 mmol, 1.2 eq) after 20 min of
heating under microwaves at 130 °C, and purified according to method 2. 2t was obtained under the form of a very
hygroscopic brown solid (39.2 mg, 95%). 1H NMR (400 MHz, MeOH-d4) δ 9.28 (d, 2H, J = 6.9 Hz), 8.82 (t, 1H, J =
6.9 Hz), 8.33 (t, 2H, J = 6.9 Hz), 7.73 (s, 1H), 7.68-7.58 (bp, 3H), 2.54 (s, 3H). 13C NMR (100 MHz, MeOH-d4) δ
147.8, 146.0, 144.5, 142.6, 133.3, 131.5, 129.6, 125.9, 122.5, 21.3. IR (neat) ν 3409, 3360, 3032, 1492, 1382, 808,
774, 717. LC-MS m/z (%) 170 (100), 171 (12). Anal calcd for C12H12ClN.5/2H2O: C, 57.49; H, 6.83; N, 5.59. Found:
C, 57.85; H, 6.47; N, 5.65.
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1-(2-hydroxyphenyl)pyridin-1-ium chloride (2u). 2u was prepared by reacting 0.4 mmol (112.4 mg) of Zincke salt 3
with 1u (85.3 mg, 0.8 mmol, 2 eq) after 30 min of heating under microwaves at 130 °C, and purified according to
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method 3. 2u was obtained under the form of a very hygroscopic yellow solid (25.5 mg, 31%). H MNR (400 MHz,
MeOH-d4) δ 9.09 (d, 2H, J = 6.8 Hz), 8.76 (t, 1H, J = 6.8 Hz), 8.27 (t, 2H, J = 6.8 Hz), 7.59 (d, 1H, J = 7.8 Hz), 7.52
(t, 1H, J = 7.8 Hz), 7.21 (d, 1H, J = 7.8 Hz), 7.10 (t, 1H, J = 7.8 Hz). 13C NMR (100 MHz, MeOH-d4) δ 152.3,
147.7, 147.7, 134.0, 132.0, 129.1, 127.2, 121.1, 118.7. IR (neat) ν 3252, 3024, 2924, 1595, 1453, 1384, 1284, 1203,
839, 776. LC-MS m/z (%) 172 (100), 173 (13). Anal calcd for C11H10ClNO.H2O: C, 58.54; H, 5.36; N, 6.21. Found:
C, 58.22; H, 5.46; N, 5.89.
1-(4-hydroxyphenyl)pyridin-1-ium chloride (2v).36 2v was prepared from 1v (26.2 mg, 0.24 mmol, 1.2 eq) after 20
min of heating under microwaves at 130 °C, and purified according to method 2. 2v was obtained under the form of a
light brown powder (26.4 mg, 63%). 1H NMR (400 MHz, MeOH-d4) δ 9.17 (dd, 2H, J = 7.8, 2.1 Hz), 8.72 (tt, 1H, J
= 7.8, 2.1 Hz), 8.24 (t, 2H, J = 7.8 Hz), 7.66 (d, 2H, J = 8.1 Hz), 7.08 (d, 2H, J = 8.1 Hz). 13C NMR (100 MHz,
MeOH-d4) δ 161.9, 147.0, 145.9, 136.4, 129.5, 126.8, 117.9. IR (neat) ν 3307, 3111, 3054, 1594, 1512, 1478, 1312,
1293, 1233, 850, 776. LC-MS m/z (%) 172 (100), 173 (12), 197 (1). Anal calcd for C11H10ClNO.6/5H2O: C, 57.62;
H, 5.45; N, 6.11. Found: C, 57.96; H, 5.37; N, 5.69.
2-((2,4-dinitrophenyl)amino)phenol (5).33,41 The salt-free fractions obtained after the purification of 2u were
collected and concentrated. 5 was isolated by chromatography on silica gel (PE/EtOAc 75/25). Recrystallization in
i-PrOH gave 5 under the form of an orange powder (41.0 mg, 19%, mp 202-204 °C). 1H NMR (400 MHz, DMSO-d6)
δ 10.01 (s, 1H), 9.95 (s, 1H), 8.90 (d, J = 2.7 Hz, 1H), 8.24 (dd, J = 9.6, 2.8 Hz, 1H), 7.30 (dd, J = 7.8, 1.6 Hz, 1H),
7.26 – 7.19 (m, 1H), 7.02 (dd, J = 8.2, 1.3 Hz, 1H), 6.93 (td, J = 7.6, 1.3 Hz, 1H), 6.88 (d, J = 9.6 Hz, 1H). 13C NMR
(100 MHz, DMSO-d6) δ 152.2, 146.9, 136.0, 130.7, 129.8, 128.5, 127.2, 124.2, 123.3, 119.7, 117.1, 116.7. IR (neat)
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ν 3364, 3295, 3098, 1619, 1585, 1494, 1282, 1129, 741, 625, 507. GC-MS m/z (%) 127 (42), 128 (31), 140 (25), 154
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Acknowledgements
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Purifications and characterizations by LC-MS were achieved by the “plateforme chromatographique
analytique et semi-préparative” of the ICMPE. N. Z. thanks the MENESR for a grant. The authors are
grateful to Tanya Farthing for proofreading the article.
Supporting information
NMR, IR and MS spectra of purified compounds 2a-v and 5.
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