1506 Journal of Natural Products, 2009, Vol. 72, No. 8
Notes
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Conversion of 3-Epivobasinol to Vobasine. To a solution of
3-epivobasinol (2, 2.0 mg) in pyridine (0.3 mL) was added CrO3 (1.0
mg), and the mixture was kept at rt for 8 h. After evaporation, the
residue was applied to a silica gel column (CHCl3/MeOH, 9:1) to give
a compound (1.6 mg) whose spectroscopic data and [R]D value were
identical with those of vobasine.17
Cytotoxic Activity. Each cell line [HL-60 (human blood premy-
elocytic leukemia), RPMI8226 (multiple myeloma), NCI-H226 (non-
small cell lung carcinoma), HCT-116 (human colon cancer), and MCF-7
(human breast adenocarcinoma) cells] was seeded onto 96-well
microtiter plates at 1 × 104 and 5 × 103 cells per well for HL-60,
RPMI8226, NCI-H226, HCT-116, and MCF-7, respectively. Cells were
preincubated for 24 h at 37 °C in a humidified atmosphere of 5% CO2.
Different concentrations of each compound (10 µL) were added to the
cultures, and then the cells were incubated at 37 °C for 48 h. On the
third day, 15 µL of MTT solution (5 mg/mL) was added into each
well of the cultured medium. After a further 2 h of incubation, 100 µL
of 10% SDS/0.01 N HCl solution was added to each well and the
formazan crystals in each well were dissolved by stirring with a pipet.
The optical density measurements were made using a micropipet reader
(Benchmark Plus microplate spectrometer, BIO-RAD) equipped with
a two-wavelengths system (550 and 700 nm). In each experiment, three
replicates of wells were prepared for each sample. The ratio of the
living cells was determined on the basis of the difference of the
absorbance between those of samples and controls. These differences
are expressed in percentage, and cytotoxic activity was indicated as an
IC50 value.
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Acknowledgment. This work was partly supported by a Grant-in-
Aid for Scientific Research from the Ministry of Education, Culture,
Sports, Science, and Technology of Japan, and a grant from the Open
Research Center Project.
1
Supporting Information Available: H and 13C NMR spectra of
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NP900115Q