Journal of the American Chemical Society
COMMUNICATION
’ ACKNOWLEDGMENT
We are grateful for support from NSF Grant MCB0919898
and assistance from Drs. Ah-Lim Tsai, Gang Wu, Mike Mal-
kowski, Alex Vecchio, Kasia Rudzka, and Alfredo Angeles-Boza.
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Figure 5. Steady-state kinetics of the oxidation of (a) h31-LA and (b)
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2
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offsetting influence of D OKM(O2), as shown in Figure 5.
2
We note that lipoxygenase (LOX) enzymes have structures
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=
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’ ASSOCIATED CONTENT
S
Supporting Information. Details of protein preparation
b
and additional kinetic data. This material is available free of
(25) Knapp, M. J.; Seebeck, F. P.; Klinman, J. P. J. Am. Chem. Soc.
2001, 123, 2931.
’ AUTHOR INFORMATION
Corresponding Author
15849
dx.doi.org/10.1021/ja2059523 |J. Am. Chem. Soc. 2011, 133, 15846–15849