Salvage of deoxyribonucleosides in plants
A. R. Clausen et al.
(Whatman International, Maidstone, UK) assay using vari-
ous radiolabelled/labelled dN substrate concentrations [35].
For determination of dNK activities in E. coli cell extracts,
3 mM NaF was added to the reaction mixture and, when dC
was used as substrate, 0.5 mM of the cytidine deaminase
inhibitor tetrahydrouridine was added. One unit (u) of dNK
activity is defined as the formation of the corresponding
9 Bressan RA, Murray MG, Gale JM & Ross CW
(1978) Properties of pea seedling uracil
phosphoribosyltransferase and its distribution in other
plants. Plant Physiol 61, 442–446.
10 Katahira R & Ashihara H (2002) Profiles of pyrimidine
biosynthesis, salvage and degradation in disks of potato
(Solanum tuberosum L.) tubers. Planta 215, 821–828.
11 Stasolla C, Loukanina N, Ashihara H, Yeung EC &
Thorpe TA (2007) Comparative studies on pyrimidine
metabolism in excised cotyledos of Pinus radiata during
shoot formation in vitro. J Plant Physiol 164, 429–441.
12 Stasolla C, Loukanina N, Ashihara H, Yeung EC &
Thorpe TA (2006) Changes of purine and pyrimidine
nucleotide biosynthesis during shoot initiation from
epicotyl explants of white spruce (Picea glauca). Plant
Sci 171, 345–354.
monophosphate product at at a rate of 1 nmolꢁminꢀ1
.
Kinetic data were evalulated by nonlinear regression analy-
sis using the Michaelis–Menten equation v = Vmax 9 [S]/
h
h
(Km + [S]) or the Hill equation v ¼ Vmax ꢂ ½Sꢃ =ðKh0:5 þ ½Sꢃ Þ
as described previously [36].
Acknowledgements
The authors acknowledge funding from Swedish
Research Council (VR), Cancerfonden (Sweden), Wen-
ner-Gren Foundation, Crafoord Foundation, Sorensen
Foundation, Fysiografen and Jubi Kinase ApS.
13 Wang C & Liu Z (2006) Arabidopsis ribonucleotide
reductases are critical for cell cycle progression, DNA
damage repair, and plant development. Plant Cell 18,
350–365.
14 Chen IP, Haehnel U, Altschmied L, Schubert I &
Puchta H (2003) The transcriptional response of
Arabidopsis to genotoxic stress – a high-density colony
array study (HDCA). Plant J 35, 771–786.
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8
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