Bioorganic Chemistry
Efficient biogenesis of Cu2O nanoparticles using extract of Camellia sinensis
leaf: Evaluation of catalytic, cytotoxicity, antioxidant, and anti-human
ovarian cancer properties
,
,*
Lei Doua, Xinxin Zhangb, Mohammad Mahdi Zangenehc d, Yi Zhanga
a Department of Gynecology, The First Hospital of China Medical University, 155 Nanjing Street, Shenyang City, Liaoning Province 110001, China
b Discipline Inspection Commission, China Medical University, No. 77 Puhe Road, Shenyang North New Area, Shenyang, Liaoning Province 110122. China
c Department of Clinical Sciences, Faculty of Veterinary Medicine, Razi University, Kermanshah, Iran
d Biotechnology and Medicinal Plants Research Center, Ilam University of Medical Sciences, Ilam, Iran
A R T I C L E I N F O
A B S T R A C T
Keywords:
At the moment, metallic nanoparticles especially copper nanoparticles are administrated for the cure of different
disorders, such as tumor and cancer. In recent years, many chemotherapeutic supplements have been formulated
by copper nanoparticles. In the present study, copper nanoparticles were prepared and synthesized in aqueous
medium using Camellia sinensis leaf extract. The as-prepared Cu2O nanoparticles was thoroughly characterized
using XRD, FT-IR, FESEM, EDX, TEM and X-ray elemental mapping techniques. The as-synthesized Cu2O/
C. sinensis NPs applied as novel nanocatalyst for the synthesis of annulated fused pyrano[2,3-d]pyrimidinones via
a one-pot, three-component condensation of a barbituric acid, aromatic aldehydes, and malonitrile or ethyl-
cyanoacetate under mild condition at 25 ◦C. Main properties of this facile method are the involves an easy work-
up procedure, avoidance of hazardous or polluting chemicals, significant yields under mild conditions, and one-
pot reaction. We assessed the anti-human ovarian cancer potentials of these nanoparticles against Caov-3, SW-
626, and SK-OV-3 cell lines. For investigating the antioxidant activities of CuCl2⋅2H2O, C. sinensis, and copper
nanoparticles, the DPPH free radical test was used. For the determining of anti-human ovarian cancer properties
of CuCl2⋅2H2O, Camellia sinensis leaf aqueous extract, copper nanoparticles, and Carboplatin (Standard positive
control), MTT assay was used on normal (HUVECs) and human ovarian cancer (Caov-3, SW-626, and SK-OV-3)
cell lines. Copper nanoparticles had high cell death and anti-human ovarian cancer properties against Caov-3,
SW-626, and SK-OV-3 cell lines. Among the above cell lines, the best result was gained in the cell line of SW-
626. According to the above findings, it looks copper nanoparticles green-synthesized by Camellia sinensis leaf
aqueous extract have the potential to be used as a chemotherapeutic material for human ovarian cancers.
Copper nanoparticles
Annulated fused pyrano[2,3-d]pyrimidinones
Camellia sinensis
Anti-human ovarian cancer properties
1. Introduction
ovary syndrome, and ovarian epithelial cancer (Ovarian cancer) [1]. The
ovarian cancer is one of the most common cancers in women in both
There is an ovary found on each side of the body. The ovary is an
organ found in the female reproductive system that produces an ovum.
When released, this travels down the fallopian tube into the uterus,
where it may become fertilized by a sperm. The ovaries also secrete
hormones that play a role in the menstrual cycle and fertility. The ovary
progresses through many stages beginning in the prenatal period
through menopause. It is also an endocrine gland because of the various
hormones that it secretes [1]. The main diseases that affect the normal
function of ovary are included endometriosis, ovarian cysts, ovarian
germ cell tumors, ovarian low malignant potential tumors, polycystic
developing and developed countries. In first, ovarian cancer arises with
growing an abnormal cell and after a while it spread in all parts of the
uterine and also all parts of the body [1,2]. Ovarian cancer is a cancer
that forms in or on an ovary. It results in abnormal cells that have the
ability to invade or spread to other parts of the body. When this process
begins, there may be no or only vague symptoms. Symptoms become
more noticeable as the cancer progresses. The main risk factors of
ovarian cancer are family history, age, reproductive history, breast
cancer, hormone therapy, obesity and overweight, gynecologic surgery,
human papillomavirus, and the use of talcum powder [1,2]. Suppression
* Corresponding author.
Received 20 July 2020; Received in revised form 3 October 2020; Accepted 7 November 2020
Available online 10 November 2020
0045-2068/© 2020 Elsevier Inc. All rights reserved.