stimulation for 30 min the media was aspirated and the cells
washed with PBS buffer (1.5 ml). The cells were fixed using
4% paraformaldehyde in PBS (1.2 ml). Confocal microscopy
experiments were carried out using a Leica DM IRE2 microscope
with a UV laser; cells were analyzed at 20-fold and 63-fold
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Acknowledgements
We thank the BBSRC (Studentship to I. A. I.), and MRC for
financial support of this work; and the Scottish Executive/Royal
Society of Edinburgh (Research Fellowship to A. N. H.). We thank
Professor Sir Philip Cohen FRS for advice on SAPK pathway
activation, Dr Simon Morton for technical assistance with the
immunoassays, and Dr Rosario M. Sanchez-Martin for assistance
with FACS analysis and microscopy.
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