Bioorganic and Medicinal Chemistry Letters p. 6062 - 6065 (2007)
Update date:2022-09-26
Topics:
Zhang, Jian
Sun, Yang
Liu, Ji-Hua
Yu, Bo-Yang
Xu, Qiang
Resibufogenin, cinobufagin, and bufalin are cytotoxic steroids isolated from the Chinese drug Chan'su. Biotransformation of these three bufadienolides by Nocardia sp. NRRL 5646 was investigated. Notably, resibufogenin was converted to 3-acetyl 15β-hydroxyl bufotalin, via an unprecedented 14β,15β-epoxy ring cleavage and a regio-selective acetoxylation. This product showed significantly increased cytotoxic activity. The regio-selective acetylation of the 3-OH was also involved in the other reactions. The structures of metabolites were established by ESI-LC/MS and 2D NMR techniques. The in vitro cytotoxic activities against human cancer cell lines of the substrates and the transformed products were determined by the MTT method and their structure-activity relationship (SAR) was discussed. This investigation provided a useful approach to prepare new bufadienolides and the SAR research.
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