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ACKNOWLEDGMENTS
■
We thank Andrew Bigley for providing the DEVX substrate,
PdPTE variants, and kinetic parameters of wild-type PdPTE for
DEVX hydrolysis. We thank Jorg Claren and Rainer Merkl for
discussion and comments on the manuscript. M.M.M. was
supported by fellowships of the German Academic Exchange
Service, the Bavarian State government, and the Fonds der
Chemischen Industrie. This work was supported in part by the
NIH (GM 68550).
̈
(33) Afriat-Jurnou, L.; Jackson, C. J.; Tawfik, D. S. Biochemistry 2012,
51, 6047.
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dx.doi.org/10.1021/ja405911h | J. Am. Chem. Soc. 2013, 135, 11670−11677