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10.1002/cplu.201700194
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were diluted in MHB in order to reach OD~ 0.6 at the wavelength of 600
nm, then they were incubated at 37° C by shaking at 300 rpm. After 30
minutes of treatment, 500 L samples were collected, centrifuged at 3.500
rpm, and the medium was removed and replaced with 1 mM PI in PBS.
The samples were incubated in the dark at room temperature for 15 min
and then centrifuged at 3.500 rpm. The supernatant was removed and
replaced with 1X PBS. Bacterial cells were visualized using an OLYMPUS
BX 41 microscope; the images were taken with CSV1.14 software using a
CAMXC-30 for image acquisition. Fluorescent signal intensity was
assessed using a fluorescent plate reader (ex. 530 nm; em.620 nm).
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Keywords: antimicrobial polymers • antifouling membranes •
organic synthesis • polymerizable quaternary ammonium salts •
quaternary ammonium salts
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