Paper
RSC Advances
ketones revealed the formation of an R-congured stereo-center
for the main product. Likewise, for its physiological substrates
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acetoin and diacetyl, this enzyme shows the same stereo- 10 K. B. Sharpless, W. Amberg, Y. L. Bennani, G. A. Crispino,
selectivity for several unnatural substrates. BcBDH belongs to
the family of zinc-dependent alcohol dehydrogenases. Because
of this, we tested the inuence of metal ions on the enzyme
J. Hartung, K. S. Jeong, H. L. Kwong, K. Morikawa,
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¨
observe a high activity boost (more than double the activity) by
supplementing this enzyme with Mn2+ ions. This effect could
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enhancing the conversion rate by using the enzyme previously 12 M. Knoll, M. Muller, J. Pleiss and M. Pohl, ChemBioChem,
pre-incubated with Mn2+ ions.
2006, 7(12), 1928–1934, DOI: 10.1002/cbic.200600277.
In summary, BcBDH is a suitable biocatalyst for the stereo- 13 R. Westphal, S. Waltzer, U. Mackfeld, M. Widmann, J. Pleiss,
¨
selective synthesis of a-hydroxy ketones and vicinal diols. With
M. Beigi, M. Muller, D. Rother and M. Pohl, Chem. Commun.,
its ability to distinguish between asymmetric and symmetric
2013, 49(20), 2061, DOI: 10.1039/c3cc38607d.
´
´
molecules, the product outcome can be predicted. Furthermore, 14 P. Hoyos, M. Fernandez, J. V. Sinisterra and A. R. Alcantara, J.
the ability of accepting “bulky” substrates and the stereo-
selective reduction of these molecules makes this enzyme even
more appealing.
Org. Chem., 2006, 71(20), 7632–7637, DOI: 10.1021/
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15 G. Scheid, W. Kuit, E. Ruijter, R. V. A. Orru, E. Henke,
U. Bornscheuer and L. A. Wessjohann, Eur. J. Org. Chem.,
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Conflicts of interest
´
16 A. Petrenz, P. D. D. Marıa, A. Ramanathan, U. Hanefeld,
There are no conicts to declare.
M. B. Ansorge-Schumacher and S. Kara, J. Mol. Catal. B:
Enzym.,
2015,
114,
42–49,
DOI:
10.1016/
j.molcatb.2014.10.011.
Acknowledgements
´
´
´
´
17 E. Calam, S. Porte, M. R. Fernandez, J. Farres, X. Pares and
J. A. Biosca, Chem.-Biol. Interact., 2013, 202(1–3), 195–203,
DOI: 10.1016/j.cbi.2012.12.006.
This project is nancially supported by the Ministry of Inno-
vation, Science, and Research of the State of North Rhine-
´
´
Westphalia. We acknowledge the assistance of Dr Alexandros 18 E. Calam, E. Gonzalez-Roca, M. R. Fernandez, S. Dequin,
´
Katranidis (Institute of Complex Systems
–
Molecular
X. Pares, A. Virgili and J. A. Biosca, Appl. Environ.
¨
Biophysics; Forschungszentrum Julich) for CD-spectroscopy
measurements, Inga Recker for helpful discussions and thank
Melanie Gellissen and Klaudia Adels for technical assistance.
Microbiol., 2016, 82(6), 1706–1721, DOI: 10.1128/
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19 R. Medici, H. Stammes, S. Kwakernaak, L. G. Otten and
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20 X. Chen, T. Mei, Y. Cui, Q. Chen, X. Liu, J. Feng, Q. Wu and
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