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RSC Advances
Page 6 of 15
DOI: 10.1039/C6RA00103C
ARTICLE
Journal Name
3.9. Thermal and pH dependence
We are grateful to the University of Kurdistan Research Councils
for partial support of this work. We are also thankful to Dr. R.
Thermal activities of the free laccase and MNPs-Laccase were Shakeri, Department of Biochemistry, University of Kurdistan, for
tested by measuring the activities after staying in Na-phosphate her valuable suggestions.
buffer at pH 6.0 at different temperatures (20–80 °C). The relative
activity was calculated as the ratio between the activity at each
References
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MNPs-Laccase were determined by measuring the activities after
they were put in different buffer solutions (pH 3–8) at 4 ºC for 5 h.
The Relative activities were determined as the ratio of the obtained
activity at the corresponding pH and the maximum attained.
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In summary, we have synthesized and characterized magnetic
core-shell nanoparticle-immobilized laccase from Trametes
Versicolor (MNPs-Laccase). The thermal, pH and storage stabilities
of MNPs-Laccase were effectively improved compared with its free
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Acknowledgment
6 | J. Name., 2012, 00, 1-3
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