3564
Z. Li et al. / Tetrahedron 69 (2013) 3561e3564
4.3. Ketone reduction catalyzed by the yeast carbonyl re-
ductase PasCR
(500 MHz, CDCl3) 3.68 (1H, dd, J¼8.5, 11.5), 3.77 (1H, dd, J¼3.0, 11.5)
4.83 (1H, dd, J¼3.5, 8.5), 7.3e7.7 (5H, m).
The typical reaction procedure was as follows:
(18 g Lꢁ1), glucose dehydrogenase (2 g Lꢁ1), NADPH (0.5 g Lꢁ1),
PasCR (0.5 g Lꢁ1), and substrate solution in DMSO (50
L, 0.25 M)
D-glucose
Acknowledgements
m
This work was financially supported by the Chinese Academy of
Sciences (KGCX2-YW-203 and KSCX2-EW-G-14) and National Key
Basic Research and Development Program (2011CB710801).
were mixed in 1 mL of phosphate buffer (100 mM, pH 6.5). The
reaction mixture was shaken at 30 ꢀC overnight and extracted by
methyl tert-butyl ether (1 mL). The organic extract was dried over
anhydrous sodium sulfate and subjected to chiral GC or HPLC to
determine the conversion and enantiomeric excess. The absolute
configurations of product alcohols were identified by comparing
the chiral GC or HPLC data with those of the standard samples. A
control experiment was performed under same conditions without
addition of the enzyme PasCR and no reduction product was
observed.
References and notes
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