Bioscience, Biotechnology and Biochemistry p. 1676 - 1679 (2001)
Update date:2022-08-29
Topics:
Saratani, Yuri
Uheda, Eiji
Yamamoto, Hiroaki
Nishimura, Atsuo
Yoshizako, Fumiki
The enantioselectivity of ECAA to ECHB by eight fungi of four genus was evaluated. All strains showed (S)-selectivity, and Cylindrocarpon sclerotigenum IFO 31855 gave the highest yield and good optical purity (e.e.; >99%). Cell-free extract and acetone-dried cells of C. sclerotigenum IFO 31855 reduced ECAA to (S)-ECHB in the presence of NADPH (e.e.; > 99%) and the e.e. was not decreased by heat treatment of the cell-free extract or the acetone-dried cells. The active fractions shown by two peaks on a DEAE-Toyopearl 650 M column gave preferentially (S)-ECHB (e.e.;>99%).
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