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kinetics, high chemoselectivity, and excellent functional group
tolerance. With this work, we expand the scope of biomolecule
modification3,5,38–40 by providing tools that interface bond form-
ing processes characteristic of organometallic complexes18,25,41–
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with bioconjugation. The straightforward synthetic proce-
dures and commercially available or otherwise easily accessible
reagents presented should expand the bioconjugation space well
beyond the substrates and peptides reported in this study. This
work also expands on a relatively underrepresented class of or-
ganometallic reagents containing metal-carbon bonds capable
of withstanding relatively harsh environmental conditions.47
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ASSOCIATED CONTENT
Supporting Information
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Falconer, R. L.; Fensham-Smith, A. J.; Gates, P. J.;
Leman, T.; Mcgrady, J. E.; Bower, J. F.; Russell, C. A. J.
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Detailed experimental procedures, characterization data, and
crystallographic data are available in the Supporting Infor-
mation. The Supporting Information is available free of charge
on the ACS Publications website.
AUTHOR INFORMATION
Corresponding Author
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Author Contributions
‡These authors contributed equally.
Notes
The authors declare no competing financial interests.
ACKNOWLEDGMENT
A.M.S. thanks UCLA Department of Chemistry and Biochemistry
for start-up funds, 3M for a Non-Tenured Faculty Award, Alfred
P. Sloan Foundation for a Fellowship in Chemistry and the Na-
tional Institutes of Health (NIH) for a Maximizing Investigators
Research Award (MIRA, R35GM124746). H.D.M thanks the Na-
tional Science Foundation (NSF, CHE-1507735) for funding.
M.S.M thanks the NSF for the Bridge-to-Doctorate (HRD-
1400789) and the Predoctoral (GRFP) (DGE-0707424) Fellow-
ships and UCLA for the Christopher S. Foote Fellowship. Dr.
Jacquelin Kammeyer (UCLA) is acknowledged for helpful discus-
sions and Mr. Nicholas Bernier (UCLA) is thanked for assistance
with ICP-AES measurements. The authors thank Dr. Chi Zhang
and Prof. Bradley L. Pentelute (MIT) for generously sharing the
plasmid used for DARPin expression and the UCLA-DOE Insti-
tute Protein Expression Technology Center (PETC) for expres-
sion of DARPin and Dr. Yu Chen (UCLA) for help with mass spec-
trometry.
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