1642
L.L. Machado et al. / Phytochemistry 67 (2006) 1637–1643
market and were identified by botanist Prof. Edson P.
Nunes. Vouchers specimens numbers: M. esculenta 34.831
and Manihot dulcis 34.830 have been deposited at the Her-
barium Prisco Bezerra of the Departamento de Biologia,
Universidade Federal do Cear a´ , Brazil.
Acknowledgments
The authors thank the Brazilian funding agencies
CNPq, CAPES, FUNCAP, and PRONEX for fellowships
and financial support. Also, we are indebted to PADE-
TEC/UFC for GC–MS and to CENAUREN/UFC, for
NMR data. The authors also thank Dr. Regina C.M. de
Paula and Dr. Jeanny S. Maciel, UFC-Brazil for assistance
in the protein determination.
3
2
.3. Bioreduction of compounds 1–9, 18–24 and hydrolysis of
5–29
3
.3.1. Extraction and isolation
The M. esculenta and M. dulcis roots were rinsed with
% sodium hypochlorite solution and sterile distilled
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(
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(
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(
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173 mg/131 mg); 32 (140 mg/135 mg); 33 (144 mg/
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stones of a patient, recrystallized with CHCl . S(-)1-phe-
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pentanol 30, cyclohexanol 31, S(À)-carveol 32, R(+)-pule-
gol 33, cholestan-3-one 23, sitoster-3-one 24, butyric acid
a
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benzamide 28, and benzonitrile 29 were fully characterized
according to their spectral data (Pouchert, 1981; Pouchert
and Behnke, 1993; Lee et al., 1996).