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Catalysis Science & Technology
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DOI: 10.1039/D0CY00912A
N. Willems, M. Lelimousin, J. Skjold-Jørgensen, A. Svendsen
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In this work, chemical modifications of lipase from
Thermomyces
lanuginosus
with
different
linkers
(isothiocyanates and aldehydes) were for the first time explored
to improve activity, stability, and affinity to differentiated
substrates. The results showed that aldehyde linkers were more
prone to modify the lysines of lipase comparing with the
isothiocyanates. One concluded that the size and hydrophobic
character of the linkers influenced greatly the enzyme activity.
The longer and hydrophobic is the linker, the stronger is its
interaction with the hydrophobic amino acid residues near the
active site, conducting to a destabilization of the lid and to an
enlargement of the active site’s cavity. This is expected to
induce an improvement of the lipase performance.
The implementation of the easy methodology developed,
broadening the substrates chain-length specificity, increments
thereafter the range of substrate possibilities that can be
hydrolysed by this lipase, paving the way to the establishment
of new industrial applications.
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Conflicts of interest
There are no conflicts to declare.
Acknowledgements
This study was supported by the Portuguese Foundation for
Science and Technology (FCT) under the scope of the strategic
funding of UIDB/04469/2020 unit and BioTecNorte operation
(NORTE-01-0145-FEDER-000004) funded by European Regional
Development Fund under the scope of Norte2020 - Programa
Operacional Regional do Norte. The authors also thanks to FCT
for funding: Jennifer Noro (SFRH/BD/121673/2016) and Carla
Silva (SFRH/IF/00186/2015). Tarsila Castro thanks the senior
position funded by the European Union through the European
Regional Development Fund (ERDF) under the Competitiveness
Operational Program (BioCell-NanoART= Novel Bio-inspired
Cellular
Nano-architectures,
POC-A1.1.4-E-2015
nr.
30/01.09.2016). Access to computing resources funded by the
Project “Search-ON2: Revitalization of HPC infrastructure of
UMinho” (NORTE-07-0162-FEDER-000086), co-funded by the
North Portugal Regional Operational Programme (ON.2 – O
Novo Norte), under the National Strategic Reference
Framework (NSRF), through the European Regional
Development Fund (ERDF), is also gratefully acknowledged.
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