1352
SHMANAI, LITOSHKO
NaIO4 and left overnight. To remove anions (iodate,
periodate, iodide), the solution was passed through a
column packed with Dowex 21-K anion-exchange
resin equilibrated with the same buffer solution;
0.5-ml fractions were collected. The presence of the
oxidized product was checked by the reaction with
DNPH (formation of intensely colored hydrazones).
The absence of periodate and iodate ions was verified
by the reaction with iodide ion (formation of free
iodine) in acid solution.
ACKNOWLEDGMENTS
The study was supported by the Belarussian Re-
publican Foundation for Basic Research (project
no. Kh98M-079). The authors are grateful to S.E. Ban-
darik for providing the possibility for immunization
and for assistance in preparing the antiserum.
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2
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RUSSIAN JOURNAL OF APPLIED CHEMISTRY Vol. 74 No. 8 2001