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New Journal of Chemistry
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Journal Name
COMMUNICATION
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mitochondria.
DOI: 10.1039/D0NJ04484A
Probe based on the quinoline ring large conjugated
architecture, which exhibited an Stokes shift of 170 nm, and
had rapid response capability and low detection limit in
aqueous phase. In biological imaging experiments, the Probe
can be well accumulated in the mitochondria and successfully
carry out internal and external experiments and mouse
imaging.
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Figure 6 The co-localization image of the cells co-cultured with the
Probe (10 μM) and Mitotracker red (1 μM). (a) Indicates the red
channel (mitochondrial dye staining); (b) Indicates yellow channel
(probe staining); (c) Indicates bright field; (d) Indicates merged
images; (e) Correlation R=0.91. (f) Intensity profile of linear region
of interest across the HeLa cell costained with Yellow channel of
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Conflicts of interest
There are no conflicts to declare.
Acknowledgments
Probe and Mitotracker Red; Red channel, λem= 660-720 nm, λex
=
We thank the National Natural Science Foundation of China (No.
21775096, 21907062, 21878180,), One hundred people plan of
Shanxi Province, Shanxi Province"1331 project" key innovation
team construction plan cultivation team (2018-CT-1), 2018
Xiangyuan County Solid Waste Comprehensive Utilization Science
and Technology Project (2018XYSDJS-05), Shanxi Province
Foundation for Returness (2017-026), Shanxi Collaborative
Innovation Center of High Value-added Utilization of Coal-related
Wastes (2015-10-B3), the Shanxi Province Foundation for Selected
(No. 2019), Innovative Talents of Higher Education Institutions of
Shanxi, Scientific and Technological Innovation Programs of Higher
Education Institutions in Shanxi (2019L0031), Key R&D Program of
Shanxi Province (201903D421069), the Shanxi Province Science
Foundation (No. 201901D111015), Key R&D and transformation
plan of Qinghai Province (No. 2020-GX-101 and Scientific
Instrument Center of Shanxi University (201512).
633 nm; Yellow channel: λem= 540-600 nm, λex = 488 nm. Scale bar:
20
μm.
3.7 The imaging of Probe in mice
Then we studied the SO2 imaging of Probe in mice. Before the
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injection of Probe and HSO3 , we photographed the blank mice
and found that there was no fluorescence signal. After injected
Probe into the abdominal cavity of mice, a strong fluorescent
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signal was observed. After intraperitoneal injection of HSO3
solution, photographs were taken at different times. It can be
seen from the Figure7c-7d that the fluorescence signal gradually
weakened after 30 min. We added an experiment, only the probe
was shot at different times when the probe was injected into the
abdominal cavity of the mouse. From Figure 7e-7h, it can be
clearly seen that within 120 min, the fluorescence signal in the
mouse's abdominal cavity is weakened to a certain extent under a
certain metabolic effect. It showed that the Probe can track SO2
directly in vivo.
References
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2
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9
J.C. Xu, H.Q. Yuan, L.T. Zeng and G.M. Bao, Chin. Chem. Lett., 2018,
29, 1456-1464
.
Y.Y. Ma, Y.H. Tang, Y.P. Zhao and W.Y. Lin, Anal. Chem., 2019, 91,
10723-10730.
Y.K. Yue, F.J. Huo, P. Ning, Y.B. Zhang, J.B. Chao, X.M. Meng and
C.X. Yin, J. Am. Chem. Soc., 2017, 139, 3181-3185.
L. Zhang, X.A. Liu, K.D. Gillis and T.E. Glass, Angew. Chem. Int. Ed.,
2019, 58, 7611-7614.
H.F. Jin, Y. Wang, X.B. Wang, Y. Sun, C.S. Tang and J.B. Du, Nitric
Oxide, 2013, 32, 56-61.
S.X. Du, H.F. Jin, D.F. Bu, X. Zhao, B. Geng and C.S. Tang, Acta
Pharmacol. Sin., 2008, 29, 923-930.
L. Luo, S. Chen, H. Jin, C. Chang and J. Du, Biochem. Bioph. Res. Co.,
2011, 415, 61-67.
X. Y. Han, X.Y. Song, F.B.Yu and L.X. Chen, Chem. Sci., 2017, 8,
6991-7002.
Figure 7. (a) Blank shooting. (b-d) After 10 min of NaHSO3 (200
μM) injection, the Probe (20 μM) was injected, and then live
fluorescence images at 0, 10, and 30 min. (e-h) After injecting, the
real-time fluorescence image of the Probe at 0, 40, 80 and 120
J. R. Stone and S. Yang, Antioxid. Redox. Signal., 2006, 8, 243-270.
10 J.W. Lee and J.D Helmann, Nature, 2006, 440, 363-367
11 F.Y. Qin, Y.R. Zhang, J.M. Zhu, Y. Li, W.B. Cao and Y. Ye
Actuators B Chem., 2019, 291, 207-215
.
,
Sens.
.
min.
λex
=
405
nm,
λem
=
500-590
nm.
12 M.Y. Li, P.C Cui, K. Li, J,H. Feng, M.M. Zou and X.Q. Yu, Chin.
Chem. Lett., 2015, 29, 992-994.
13 Y. Zhou, F.W. Zeng, Y. Liu, M. Li, S.Z.Xiao, J.Y. Yan, N.N. Zhang
and K.B. Zheng, Tetranedron Lett., 2018, 59, 3253-3257.
3. Conclusion
This journal is © The Royal Society of Chemistry 20xx
J. Name., 2013, 00, 1-3 | 5
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