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18
surface plasmon resonance was shown to be a tetrasaccharide.
The same heparin tetrasaccharide, after tagging with a terminal
fluorophore (2-aminoacridone), retained its HGF/SF-binding
1
9
ability by gel mobility shift assay. Hence we anticipate that in
solution phase GAG–protein complexes, the paramagnetic
relaxation enhancement induced by the electron radical in 12c
on protein protons will lead to generation of important distance
restraints.
1
998, 6, 109–116.
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demonstrated. We are currently investigating the application of
this methodology to conformational studies of GAGs using FRET
and NMR.
8
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We thank the EPSRC (Grant Ref. EP/C539001) for financial
support of this work, Jon Deakin for technical HPLC advice, and
the Scottish Executive/Royal Society of Edinburgh (Research
Fellowship to A. N. H.).
1
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{
Experimental procedure for the DMT-MM mediated coupling: 50 mg of
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with the appropriate amine (1.2 eq.) and the mixture was stirred at room
temperature for 10 min. DMT-MM (1.5 eq.) was then added and the
reaction stirred at room temperature until complete (5–14 h). The solvent
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688 | Chem. Commun., 2007, 2686–2688
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