Nucleosides and Nucleotides p. 937 - 939 (1997)
Update date:2022-08-16
Topics:
Picken, Douglas
Gault, Vicki
Enzyme catalysis by a cell free preparation from Lactobacillus leichmannii is used to accomplish the transformation of thymidine to deoxynebularine in the presence of purine. The resulting mixture of nucleosides is then converted to their 5'-dimethoxytrityl derivatives which are easily separated using conventional chromatography. Phosphitylation of the 5'-dimethoxytrityl-deoxynebularine then gives the phosphoramidite derivative suitable for incorporation into oligonucleotides.
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