Catalytic activity of baker’s yeast in ester hydrolysis 291
Glanzer BI, Faber K, Griengl H. 1987. Enantioselective
hydrolyses by baker’s yeast – II: esters of n-acetyl amino acids.
Tetrahedron 43:771–778.
culture medium composition has been observed
(Dalmau et al. 2000). Valero et al. (1991) reported
that lipase production was sensitive to glucose
repression, but Chang et al. (1994) showed that
lipase was produced in the presence of glucose.
Ham HJ, Rho HJ, Shin KS,Yoon H-J. 2010. The TGL2 gene of
Saccharomyces cerevisiae encodes an active acylglycerol lipase
located in the mitochondria. J Biol Chem 285:3005–3013.
Jandrositz A, Petschnigg J, Zimmermann R, Natter K, Scholze H,
Hermetter A, Kohlwein SD, Leber R. 2005. The lipid droplet
protein Tgl1p hydrolyzes both steryl esters and triglycerides in
the yeast Saccharomyces cerevisiae. Biochem Biophys Acta
1735:50–58.
Kanno O, Kawamoto I. 2000. Stereoselective synthesis of novel
anti-MRSA tricyclic carbapenems (trinems). Tetrahedron 31:
5639–5648.
Koffel R, Tiwari R, Falquet L, Schneiter R. 2005. The
Saccharomyces cerevisiae YLL012/YEH1, YLR020/YEH2, and
TGL1 genes encode a novel family of membrane-anchored
lipases that are required for steryl ester hydrolysis. Mol Cell
Biol 25:1655–1668.
Conclusions
Our experiments have provided further evidence
for the possibility of lipase production by baker’s
yeast. It appears that it is induced during growth of
baker’s yeast and probably depends on culture
medium composition, as has been observed for
other yeast strains. This may also be true of other
yeasts considered to be non-lipolytic strains.
Reetz MT. 2000. Lipases as practical biocatalysts. Curr Opin
Chem Biol 6:145–150.
Roberts S (editor). 1999. Biocatalysis for fine chemicals synthesis.
Chichester: Wiley.
Declaration of interest: The authors report no
conflicts of interest. The authors alone are respon-
sible for the content and writing of the paper.
Rodriguez S, Kayser MM, Stewart JD. 2001. Highly stereoselec-
tive reagents for β-keto ester reductions by genetic engineering
of baker’s yeast. J Am Chem Soc 123:1547–1555.
SatoT, FujisawaT. 1990. Stereocontrol in bakers’ yeast reduction
leading to natural product synthesis. Biocatal Biotransforma-
tion 3:1–15.
References
Athenstaedt K, Daum G. 2005.Tgl4p andTgl5p, two triacylglyc-
erol lipases of the yeast Saccharomyces cerevisiae are localized
to lipid particles. J Biol Chem 280:37301–37309.
Białecka-Florjan´czyk E, Majewska E. 2010. Chemoselective
hydrolysis of ester bonds in the presence of baker’s yeast. Synth
Commun 40:1264–1269.
Chang RC, Chou SJ, Show JF. 1994. Multiple forms and func-
tions of Candida rugosa lipase. Biotechnol Appl Biochem 19:
93–97.
Chin-Joe I, Nelisse PM, Straathof AJJ, Jongejan AJ, Pronk JT,
Heijnen JJ. 2000. Hydrolytic activity in baker’s yeast limits the
yield of asymmetric 3-oxo ester reduction. Biotechnol Bioeng
69:370–376.
Csuk R, Glanzer BI. 1991. Baker’s yeast mediated transforma-
tions in organic chemistry. Chem Rev 91:49–97.
Dalmau E, Montesinos J, Lotti M, Casas C. 2000. Effect of dif-
ferent carbon sources on lipase production by Candida rugosa.
Enzyme Microb Technol 26:657–663.
Schousboe I. 1976. Properties of triacylglycerol lipase in a mito-
chondrial fraction from baker’s yeast (Saccharomyces cerevisiae).
Biochim Biophys Acta 450:165–174.
Servi S. 1990. Baker’s yeast as a reagent in organic synthesis.
Synthesis 1:1–25.
Shirazi SH, Rahman SR, Rahman MM. 1998. Production
of extracellular lipases by Saccharomyces cerevisiae. World J
Microbiol Biotechnol 14:595–597.
Stahl G, Ben Salem SN, Chen L, Zhao B, Farabaugh PJ. 2004.
Translational accuracy during exponential, postdiauxic, and
stationary growth phases in Saccharomyces cerevisiae. Eukaryotic
Cell 3:331–338.
Sybesma WFH, Straathof AJJ, Jongejan AJ, Pronk JT, Heijnen JJ.
1998. Reductions of 3-oxo esters by baker’s yeast: current
status. Biocatal Biotransformation 16:95–134.
Taketani S, NishinaT, Katsuku H. 1981. Purification and proper-
ties of sterol-ester hydrolase from Saccharomyces cerevisiae. J
Biochem 89:1667–1673.
D’Arrigo P, Högberg H-E, Pedrocchi-Fantoni G, Servi S. 1994.
Old and new synthetic capacities of baker’s yeast. Biocatal
Biotransformation 9:299–312.
Degrassi G, Uotila L, Klima R, Venturi V. 1999. Purification and
properties of an esterase from the yeast Saccharomyces cerevisiae
and identification of the encoding gene. Appl Environ Microbiol
65:3470–3472.
Vakhlu J, Kour A. 2006.Yeast lipases: enzyme purification, biochem-
ical properties and gene cloning. Electron J Biotechnol 9:69–85.
Valero F, Del Rio JL, Poch M, Sola C. 1991. Fermentation
behaviour of lipase production by Candida rugosa growing on
different mixtures of glucose and olive oil. J Ferment Bioeng
72:399–401.
Wheeler GE, Rose AH. 1973. Location and properties of an
esterase activity in Saccharomyces cerevisiae. J Gen Microbiol
74:189–192.
Gill J, Parish J. 1997. Lipases – enzymes at an interface. Biochem
Educ 25:2–5.