4376 J ournal of Medicinal Chemistry, 2003, Vol. 46, No. 21
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best, modest effects toward FAAH regardless as to
whether Triton X-100 was present in the assay and
regardless of the assay pH. There was also little effect
of 14 and 15 on the uptake of AEA into RBL-2H3 cells.
In conclusion, this study has demonstrated that the
sensitivity of NPAA to inhibition by ester derivatives
of PEA, as well as by retroamide and retroester com-
pounds, is different from that for FAAH. Such com-
pounds may be very useful in delineating the physi-
ological role played by this enzyme.
Ack n ow led gm en t. The authors are grateful to Britt
J acobsson for excellent technical assistance. These
studies were supported by grants from the Swedish
Research Council (Grant no. 12158, medicine), the
Swedish Asthma and Allergy Association’s Research
Foundation, Konung Gustav V’s and Drottning Victorias
Foundation, the Swedish Psoriasis Association, the
Research Funds of the Medical Odontological Faculty,
Umeå University, the Belgian National Fund for Sci-
entific Research, and an FSR grant from the Universite´
catholique de Louvain.
Su p p or tin g In for m a tion Ava ila ble: Spectroscopic data
(TLC, mp, 1H NMR, 13C NMR, and mass spectrometry) for
compounds 5-15. This material is available free of charge via
the Internet at http://pubs.acs.org.
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