66
C. Cui et al. / Journal of Molecular Catalysis B: Enzymatic 91 (2013) 59–66
observed and this loss reached 47% after 23 cycles. This was possi-
bly due to the inactivation of lipases and the water accumulation
on the immobilization support [56]. However, the immobilized
lipase on PEI-coated support showed a more excellent stability
for esterification, maintaining a high ester yield around 90% even
after 30 cycles. The results imply that the operational stability of
immobilized Y. lipolytica lipase was greatly improved by covalently
by the immobilization methods and supports structure, and by
the micro-environment of the support, such as active functional
the enzyme and solid support will not only increase the binding
points but also provide a proper micro-environment for the enzyme
immobilization, resulting in enhancement in operational stability
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In this study, a polyethyleneimine (PEI)-coated polyurethane
foam (PUF) was used to immobilize lipase Lip2 from Y. lipolytica.
PUF shows some attractive potential for enzyme immobilization,
such as hydrophobic, high porosity, low cost, good stability and
resistance to compression deformation for the application in indus-
trial reactors. PEI coated on the PUF form a polymeric bed, not only
facilitating the immobilization but also generating a more appro-
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way of adding the GA has a significant efficient on the enzyme prop-
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was 100 mg/g PUF. Lipase Immobilized on PEI-coated support by
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nificantly enhanced thermal stability compared to free enzyme and
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This work was supported by the National Basic Research Pro-
gram of China (973 program) (2013CB733600, 2012CB725200,
2011CB710800 and 2009CB724703), the National Nature Science
Foundation of China (21106005), Key Projects in the National
Science & Technology Pillar Program during the 12th Five-year
Plan Period (2011BAD22B04), National High-Tech R&D Program of
China (863 Program) (2012AA021404 and 2011AA02A205).
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