the reaction was finished, the mixture was diluted with H O and extracted with Et O (4 ꢀ 50 mL). The Et O extracts were
2
2
2
washed by HCl solution (10%, 2 ꢀ 50 mL) to remove Py and by H O until neutral and dried over anhydrous Na SO . The
2
2
4
yield of product of 85% purity (according to HPLC) was 85% of theoretical. The product was purified by chromatography
over silica gel impregnated with AgNO (5%) and by subsequent crystallization from EtOH. The HPLC-pure product had
3
mp 42–43°C; lit. [27] mp 42.4°C.
ACKNOWLEDGMENT
We thank staff members of LPMR, Vorozhtsov NIOC, SB, RAS for GC–MS analyses of the samples. The work was
supported by Grant No. FA-A12-T117 RUzb “Creation of New Technologies of Highly Effective Drugs Based on Polyprenols
and the Study of Immunomodulator Activity.”
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