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ChemComm
COMMUNICATION
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DOI: 10.1039/D0CC01606C
performed the experiments and analysed the results. PB and VF
performed the crystallographic data collection and analysis. DD
wrote the paper with SKH and PPM.
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Figure 5 Mitochondrial localization of Cyt C by fluorescence imaging in DL cells. DL cells
were incubated with 10μg mL−1 M1 for 1h, 1μM StS for 1.5h and mito@tracker for 20
min. (A) images immediately after the StS addition and (B) images after the indicated
treatment. (1, 6) Bright filed, (2, 7) Red channel, (3, 8) green channel, (4, 9) merged, (5,
10) overlaid. Magnification (400×)
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In conclusion, For the first time, direct visualization of Cyt C
translocation event during apoptotic process is achieved using
a single crystal X-ray structurally characterized polymeric Cu(II)
complex (M1) of amide-imine conjugate through fluorescence
imaging. Moreover, M1 spatially and selectively interacts with
Cyt C leading to “turn-on” fluorescence imaging of intracellular
translocation events in live cells. Thus, M1 offers improved
detection, sensitivity, response rate as well as cellular
internalization efficiency and large signal-to-background ratio
for in vitro and intracellular quantification of Cyt C. Real-time
monitoring of Cyt C release using M1 clearly indicates that Cyt
C release is rapid and complete during cell death. In virtue of
these advantages and potentials, the developed low-cost
sensor may provide an alternative device for apoptotic studies
and catalyze the fundamental interrogations of Cyt C mediated
biology.
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Conflicts of interest
“There are no conflicts to declare”.
Notes and references
‡ Acknowledgement
SK (09/025(0227)/2017-EMR-I) and AP (09/025(0243)/2018-
EMR-I) acknowledge CSIR (New Delhi) for fellowship. SKH
acknowledges DHESTBT, Govt. WB, (No. ST/P/S& T/2G-24/2017)
for partial financial support. The SC-XRD facility is supported by
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the
project
CICECO-Aveiro
Institute
of
Materials, UIDB/50011/2020 & UIDP/50011/2020, financed by
national funds through the FCT/MEC and when appropriate co-
financed by FEDER under the PT2020 Partnership Agreement.
§§ Author contributions
4 | J. Name., 2012, 00, 1-3
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