Immobilised P. abies Cells Catalyse Transformation of ꢀ-Pinene
325
ꢁ
ꢁ
ꢁ
ꢁ
temperature program was 50 C (1 min)–40 C/min–
8
2
Microbial oxidation of ꢀ-pinene by Serratia marcescens.
Appl. Microbiol. Biotechnol., 23, 224–227 (1986).
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terpenoid hydrocarbons by basidiomycetes. In ‘‘Trends
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pp. 503–507 (1994).
ꢁ
ꢁ
0 C (15 min)–4 C/min–160 C (12 min)–20 C/min–
ꢁ
50 C (10 min), and the carrier gas was helium (0.7 ml/
min flow). Enantioselective separation was done in a
permethylated ꢁ-cyclodextrin column (HP-Chiral,
30 m ꢂ 0:25 mm, 0.25 mm film thickness), using an
HP6850 instrument (Agilent Technologies). The tem-
1
0) Hagedorn, S., and Kaphammer, B., Microbial biocatal-
ysis in the generation of flavor and fragrance chemicals.
Annu. Rev. Microbiol., 48, 773–800 (1994).
ꢁ
ꢁ
ꢁ
perature program was 40 C (1 min)–50 C/ min–60 C
ꢁ
ꢁ
ꢁ
(
ꢁ
13 min)–10 C/min–110 C (12 min)–10 C/min–150
C (20 min), and the carrier gas was hydrogen (2 ml/
11) Agrawal, R., Deepika, N.-U.-A., and Joseph, R., Strain
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min constant flow). The quantitative data shown in the
figures and tables are each the relative percentage based
on the GC integrated area.
1
2) Corbier, B., and Ehret, C., ‘‘Flavors and Fragrances:
A World Perspective’’, Proceedings 10th International
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Acknowledgments
2
0 November, Washington, DC, U.S.A., eds. Lawrence,
This work was supported by the Grant Agency of the
Academy of Sciences of the Czech Republic (project no.
IAA4055001) and gratefully acknowledged. The re-
search was performed within the framework of project
no. Z4 055 905 (Academy of Sciences of the Czech
Republic). The authors thank Dr. J. Doubsky (IOCB,
Prague) for the synthesis of p-cymen-8-ol, Dr. M.
Lindmark (Mid Sweden Univeristy, Sundsvall) for
standard samples of myrtenol and isopinocamphone,
and Ms. Z. Hornychova and Ms A. Nekolova for their
careful technical assistance.
R. M., Mookherjee, B. D., and Willis, B. J., Elsevier
Science Publishers B. V., Amsterdam, pp. 731–741
(
1986).
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