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RBM14C14 and RBM14C16, although both appear to be
poor substrates.
In summary, besides AC, NC, ACER3, and NAAA also
hydrolyze fluorogenic RBM14 compounds. Although the
selectivity of the substrates toward ceramidases can be
modulated by the length of the N-acyl chain, none of them
is specific for a particular enzyme, except for RBM14C8,
which seems to be accepted only by the ectopically ex-
pressed mNC. Despite the lack of specificity, the excellent
Z factors obtained for the assays with both NC and ACER3
warrant the suitability of these substrates in high-throughput
library screening programs aimed at identifying potent
and selective inhibitors of NC and ACER3, which are cur-
rently unknown.
The authors thank Dr. Marta Casado for her help with qPCR
and Professor Natsuo Ueda for the kind gift of pcDNA3.1(+)
and pcDNA3.1(+)-NAAA plasmids.
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trissamine (jaspine B) and diastereoisomeric jaspines. Bioorg. Med.
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Journal of Lipid Research Volume 56, 2015