Journal of Medicinal Chemistry p. 8231 - 8249 (2020)
Update date:2022-08-16
Topics:
Dubois, Lilian
Pietrancosta, Nicolas
Cabaye, Alexandre
Fanget, Isabelle
Debacker, Cécile
Gilormini, Pierre-André
Dansette, Patrick M.
Dairou, Julien
Biot, Christophe
Froissart, Roseline
Goupil-Lamy, Anne
Bertrand, Hugues-Olivier
Acher, Francine C.
Mccort-Tranchepain, Isabelle
Gasnier, Bruno
Anne, Christine
Sialin, encoded by the SLC17A5 gene, is a lysosomal sialic acid transporter defective in Salla disease, a rare inherited leukodystrophy. It also enables metabolic incorporation of exogenous sialic acids, leading to autoantibodies against N-glycolylneuraminic acid in humans. Here, we identified a novel class of human sialin ligands by virtual screening and structure-activity relationship studies. The ligand scaffold is characterized by an amino acid backbone with a free carboxylate, an N-linked aromatic or heteroaromatic substituent, and a hydrophobic side chain. The most potent compound, 45 (LSP12-3129), inhibited N-acetylneuraminic acid 1 (Neu5Ac) transport in a non-competitive manner with IC50 ≈ 2.5 μM, a value 400-fold lower than the KM for Neu5Ac. In vitro and molecular docking studies attributed the non-competitive character to selective inhibitor binding to the Neu5Ac site in a cytosol-facing conformation. Moreover, compound 45 rescued the trafficking defect of the pathogenic mutant (R39C) causing Salla disease. This new class of cell-permeant inhibitors provides tools to investigate the physiological roles of sialin and help develop pharmacological chaperones for Salla disease.
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