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Fig. 4 Signals of the proposed biosensor at different interferents and
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4. Conclusions
In summary, a fast and sensitive method for GPT activity assay
based on the uorescence quenching of BSA was developed. It
has been found that GPT effectively destroyed Cu–Ala complex
to release free Cu(II) ion, which subsequently combined with
BSA leading to the uorescence quenching. Various parameters
of this proposed method has been extensively optimized. The
superior performance of this technique including low LOD (3 U
Lꢀ1), wide working range (5–400 U Lꢀ1) and high selectivity
would pave the way for ultimately realizing the rapid and
simplied diagnosis of GPT or GPT-related diseases.
Conflict of interest
The authors have declared no conict of interest.
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Acknowledgements
We gratefully acknowledge the nancial support from National
Natural Science Foundation of China (21175155, 21475152),
and the Specialized Research Fund for the Doctoral Program of
Higher Education of China (20130162110017).
This journal is © The Royal Society of Chemistry 2015
RSC Adv., 2015, 5, 103557–103562 | 103561