10.1002/cbic.201900424
ChemBioChem
FULL PAPER
72 nmol; 5f, 22 nmol; 5g, 1.2 nmol; 5h, 21 nmol; 5i, 4 nmol: 5j and 5k, 0
nmol. These experiments were run on four different occasions and on each
occasion cultures fed with H2MAP 5a were included as the positive control.
The amounts of prodigiosin 1 produced varied (from 7 to 347 nmol) and
the production of prodigiosin analogues shown in Fig. 6 is relative to the
prodigiosin produced in the parallel cultures fed with 5a.
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Ehrlich’s assay: Solution A (MES pH 6.0 10 mM, HapB 29 µg.ml-1) and B
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corr
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A555
= A555 - (A460+A650)/2 as the pigment 17 derived by reaction of
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We acknowledge the Frances and Augustus Newman foundation,
the Cambridge Commonwealth Trust, Emmanuel College,
Cambridge, and the B.B.S.R.C. (award codes BB/N008081/1 and
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Keywords: Prodigiosin • dihydropyrrole • synthesis • FAD-
dependent oxidase • mutasynthesis
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