Journal of the American Oil Chemists' Society p. 1397 - 1403 (1994)
Update date:2022-08-11
Topics:
Kosugi, Yoshitsugu
Azuma, Naoki
More than 95percent of polyunsaturated acid (PUFA) was converted to triacylglycerol by immobilized lipase from Candida antarctica or Rhizomucor miehei.The esterification was carried out at 50-60 deg C with shaking and dehydration for 24 h.The substrates consisted of glycerol and free fatty acid or ethyl esters of the fatty acid at a 1:3 molar ratio.The docosahexaenoic acid (DHA) or eicosapentaenoic acid (EPA) in the substrate polymerized during the reaction, and they required 5-10percent more than the stoichiometric amount to compensate for the PUFA loss.On the contrary, ethyl esters of DHA and EPA were not polymerized.Pure tridocosahexaenoyl, trieicosapentaenoyl and triarachidonoyl glycerol were isolated after passing the product through a basic aluminum oxide column.Industrial feasibility of this process was discussed for the ethyl ester as substrate. KEY WORDS: Docosahexaenoic acid, docosahexaenoyl ethyl ester, eicosapentaenoic acid, immobilized lipase, lipase, polyunsaturated fatty acid, triacylglycerol, triarachidonoyl glycerol, tridocosahexaenoyl glycerol, trieicosapentaenoyl glycerol.
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