Bioscience, Biotechnology and Biochemistry p. 392 - 395 (1997)
Update date:2022-08-10
Topics:
Ohrui
Itoh
Nishida
Horie
Meguro
A model tripeptide, Gly-L-Leu-L-Phe, was immobilized with activated aminomethyl polystyrene, and its C-terminal was reduced to an alcohol. This peptidyl alcohol was selectively hydrolyzed at the C-terminal amide bond to afford a polymer-supported dipeptide (Gly-L-Leu) and amino alcohol (Phe-OH). The amino alcohol, including its absolute configuration, was determined by labelling with (+)-MNB-COOH, and the dipeptide was reused for a determination of its C-terminal amino acids. The D,L-amino acids of the tripeptide were sequentially determined from the C-terminus.
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