1446
M. HASSAN et al.
and Hanson, A. D., Choline monooxygenase, an unusual
for substrate binding and specificity in choline oxidase.
Arch. Biochem. Biophys., 430, 264–273 (2004).
17) Traynelis, V. J., and Dadura, J. G., Pyrolysis of gamma-
hydroxyalkyl quaternary ammonium hydroxides and
alkoxides: acyclic systems. J. Org. Chem., 26, 686–691
(1961).
18) Lowry, O. H., Rosebrough, N. J., Farr, A. L., and
Randall, R. J., Protein measurement with the folin
phenol reagent. J. Biol. Chem., 193, 265–275 (1951).
19) Davis, B. J., Disc electrophoresis. II. Method and
application to human serum proteins. Ann. NY Acad.
Sci., 121, 404–427 (1964).
iron-sulfur enzyme catalyzing the first step of glycine
betaine synthesis in plants: prosthetic group character-
ization and cDNA cloning. Proc. Natl. Acad. Sci. USA,
94, 3454–3458 (1997).
5)
Russell, B. L., Rathinasabapathi, B., and Hanson, A. D.,
Osmotic stress induces expression of choline monoox-
ygenase in sugar beet and amaranth. Plant Physiol., 116,
859–865 (1998).
6
)
)
Landfald, B., and Strom, A. R., Choline-glycine betaine
pathway confers a high level of osmotic tolerance in
Escherichia coli. J. Bacteriol., 165, 849–855 (1986).
Nagasawa, T., Mori, N., Tani, Y., and Ogata, K.,
Characterization of choline dehydrogenase of Pseudo-
monas aeruginosa A-16. Agric. Biol. Chem., 40, 2077–
7
20) Laemmli, U. K., Cleavage of structural proteins during
the assembly of the head of bacteriophage T4. Nature,
227, 680–685 (1970).
2
084 (1976).
21) Kaufman, R. A., and Broquist, H. P., Biosynthesis of
carnitine in Neurospora crassa. J. Biol. Chem., 252,
7437–7439 (1977).
22) Ditlow, C. C., Holmquist, B., Morelock, M. M., and
Vallee, B. L., Physical and enzymatic properties of a
class II alcohol dehydrogenase isozyme of human liver:
ꢁ-ADH. Biochemistry, 23, 6363–6368 (1984).
23) Hoshino, T., Ishiguro, I., and Ohta, Y., Rabbit liver
alcohol dehydrogenase: purification and properties.
J. Biochem., 97, 1163–1172 (1985).
8
9
)
)
Ikuta, S., Imamura, S., Misaki, H., and Horiuti, Y.,
Purification and characterization of choline oxidase from
Arthrobacter globiformis. J. Biochem. (Tokyo), 82,
1741–1749 (1977).
Rozwadowski, K. L., Khachatourians, G. G., and
Selvaraj, G., Choline oxidase, a catabolic enzyme in
Arthrobacter pascens, facilitates adaptation to osmotic
stress in Escherichia coli. J. Bacteriol., 173, 472–478
(
1991).
1
0) Yamada, H., Mori, N., and Tani, Y., Properties of
choline oxidase of Cylindrocarpon didymum M-1. Agric.
Biol. Chem., 43, 2173–2177 (1979).
1) Boch, J., Nau-Wagner, G., Kneip, S., and Bremer, E.,
Glycine betaine aldehyde dehydrogenase from Bacillus
subtilis: characterization of an enzyme required for the
synthesis of the osmoprotectant glycine betaine. Arch.
Microbiol., 168, 282–289 (1997).
2) Nagasawa, T., Kawabata, Y., Tani, Y., and Ogata, K.,
Purification and characterization of betaine aldehyde
dehydrogenase from Pseudomonas aeruginosa A-16.
Agric. Biol. Chem., 40, 1743–1749 (1976).
3) Mori, N., Kawakami, B., Hyakutome, K., Tani, Y., and
Yamada, H., Characterization of betaine aldehyde de-
hydrogenase from Cylindrocarpon didymum M-1. Agric.
Biol. Chem., 44, 3015–3016 (1980).
24) Bogin, O., Peretz, M., and Burstein, Y., Thermoanaer-
obacter brockii alcohol dehydrogenase: characterization
of the active site metal and its ligands amino acids.
Protein Sci., 6, 450–458 (1997).
1
25) Guagliardi, A., Martino, M., Iaccarino, I., De Rosa, M.,
Rossi, M., and Bartolucci, S., Purification and character-
ization of the alcohol dehydrogenase from a novel strain
ꢀ
of Bacillus stearothermophilus growing at 70 C. Int. J.
Biochem. Cell Biol., 28, 239–246 (1996).
1
1
1
1
1
26) Hirano, J., Miyamoto, K., and Ohta, H., Purification and
characterization of the alcohol dehydrogenase with a
broad substrate specifity originated from 2-phenyletha-
nol-assimilating Brevibacterium sp. KU 1309. J. Biosci.
Bioeng., 100, 315–322 (2005).
27) Hirakawa, H., Kamiya, N., Kawarabayashi, Y., and
Nagamune, T., Properties of an alcohol dehydrogenase
from the hyperthermophilic archaeon Aerpyrum pernix
K1. J. Biosci. Bioeng., 97, 202–206 (2004).
28) Narrod, S. A., and Jakoby, W. B., Metabolism of
ethanolamine. An ethanolamine oxidase. J. Biol. Chem.,
239, 2189–2193 (1964).
29) Johansson, K., El-Ahmad, M., Ramaswary, S.,
Hjelmqvist, L., Johnvall, H., and Eklund, H., Structure
4) Mori, N., Yoshida, N., and Kitamoto, Y., Purification
and properties of betaine aldehyde dehydrogenase from
Xanthomonas translucens. J. Ferment. Bioeng., 73, 352–
356 (1992).
5) Mori, N., Fuchigami, S., and Kitamoto, Y., Purification
and properties of betaine aldehyde dehydrogenase with
high affinity for NADP from Arthrobacter globiformis.
J. Biosci. Bioeng., 93, 130–135 (2002).
6) Gadda, G., Powell, N. L., and Menon, P., The trimethyl-
ammonium headgroup of choline is a major determinant
˚
of betaine aldehyde dehydrogenase at 2.1-A resolution.
Protein Sci., 7, 2106–2117 (1998).