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Laboratory Standards guidelines (1999). An inoculum density of
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at final concentrations of 25
lg/mL. Serial doubling dilutions of
norfloxacin ranging from 1 to 512
lg/mL were added and the
microtitre plates were then interpreted, after inoculum addition
and incubation, in the same manner as MIC determinations as pre-
viously described (Pereda-Miranda et al., 2006b).
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Stoloniferins
I–VII,
resin
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from
Ipomoea
stolonifera.
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various concentrations of the test samples (0.16–20 lg/mL) and
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CO2. The cell concentration was determined by the NCI sulforhoda-
mine method (Skehan et al., 1990). Results were expressed as the
dose that inhibits 50% control growth after the incubation period
(EC50). The values were estimated from a semilog plot of the drug
concentration (lg/mL) against the percentage of viable cells.
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Acknowledgements
This research was partially supported by Dirección General de
Asuntos del Personal Académico, UNAM (IN208307 and IN2173
10) and CONACyT (101380-Q). C.E.-M. and S.C.-M. are grateful to
CONACyT for graduate student scholarships. Thanks are due to G.
Duarte and M. Guzmán (USAI, Facultad de Química, UNAM) for
the recording of mass spectra.
Pongprayoon, U., Baeckström, P., Jacobsson, U., Lindström, M., Bohlin, L., 1992.
Antispasmodic activity of b-damascenone and E-phytol isolated from Ipomoea
pes-caprae. Planta Med. 58, 19–21.
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