References
[1] I.A. Mikhailopulo, A.I. Miroshnikov, New trends in nucleoside biotechnology, Acta naturae, 2 (2010) 36-59.
[2] D.D. Jordheim L. P., Zoulim F.,Dumontet C., Advances in the development of nucleoside and nucleotide
analogues for cancer and viral diseases, Nat Rev Drug Discov, 12 (2013) 447-464.
[3] E. Ichikawa, K. Kato, Sugar-modified nucleosides in past 10 years, a review, Curr Med Chem, 8 (2001) 385-
423.
[4] A. Fresco-Taboada, I. de la Mata, M. Arroyo, J. Fernandez-Lucas, New insights on nucleoside 2'-
deoxyribosyltransferases: a versatile biocatalyst for one-pot one-step synthesis of nucleoside analogs, Appl
Microbiol Biotechnol, 97 (2013) 3773-3785.
[5] R. Anand, P.A. Kaminski, S.E. Ealick, Structures of purine 2'-deoxyribosyltransferase, substrate complexes,
and the ribosylated enzyme intermediate at 2.0 A resolution, Biochemistry, 43 (2004) 2384-2393.
[6] K. Okuyama, S. Shibuya, T. Hamamoto, T. Noguchi, Enzymatic synthesis of 2'-deoxyguanosine with
nucleoside deoxyribosyltransferase-II, Bioscience, biotechnology, and biochemistry, 67 (2003) 989-995.
[7] P.A. Kaminski, Functional cloning, heterologous expression, and purification of two different N-
deoxyribosyltransferases from Lactobacillus helveticus, J Biol Chem, 277 (2002) 14400-14407.
[8] J. Fernandez-Lucas, C. Acebal, J.V. Sinisterra, M. Arroyo, I. de la Mata, Lactobacillus reuteri 2'-
deoxyribosyltransferase, a novel biocatalyst for tailoring of nucleosides, Appl Environ Microbiol, 76 (2010) 1462-
1470.
[9] C.N. Britos, V.A. Cappa, C.W. Rivero, J.E. Sambeth, M.E. Lozano, J.A. Trelles, Biotransformation of
halogenated 2′-deoxyribosides by immobilized lactic acid bacteria, Journal of Molecular Catalysis B: Enzymatic,
79 (2012) 49-53.
[10] R.K. Singh, M.K. Tiwari, R. Singh, J.K. Lee, From protein engineering to immobilization: promising
strategies for the upgrade of industrial enzymes, Int J Mol Sci, 14 (2013) 1232-1277.
[11] P.S. Liu, A.; Chu, C. K., Fluorinated Nucleosides: Synthesis and Biological Implication, J Fluor Chem, 129
(2008) 743-766.
[12] P. Liu, A. Sharon, C.K. Chu, Fluorinated nucleosides: Synthesis and biological implication, Journal of
fluorine chemistry, 129 (2008) 743-766.
[13] S.M. Park, H. Yang, S.K. Park, H.M. Kim, B.H. Kim, Design, synthesis, and anticancer activities of novel
perfluoroalkyltriazole-appended 2'-deoxyuridines, Bioorg Med Chem Lett, 20 (2010) 5831-5834.
[14] C.W. Rivero, C.N. Britos, M.E. Lozano, J.V. Sinisterra, J.A. Trelles, Green biosynthesis of floxuridine by
immobilized microorganisms, FEMS Microbiol Lett, 331 (2012) 31-36.
[15] D. Vivian, J.E. Polli, Synthesis and in vitro evaluation of bile acid prodrugs of floxuridine to target the liver,
Int J Pharm, 475 (2014) 597-604.
[16] V.A. Cappa, C.W. Rivero, C.N. Britos, L.M. Martinez, M.E. Lozano, J.A. Trelles, An efficient biocatalytic
system for floxuridine biosynthesis based onLactobacillus animalis ATCC 35046 immobilized in Sr-alginate,
Process Biochemistry, 49 (2014) 1169–1175.
[17] Y. Miyamoto, T. Masaki, S. Chohnan, Characterization of N-deoxyribosyltransferase from Lactococcus lactis
subsp. lactis, Biochim Biophys Acta, 1774 (2007) 1323-1330.
[18] J. Fernandez-Lucas, A. Fresco-Taboada, C. Acebal, I. de la Mata, M. Arroyo, Enzymatic synthesis of
nucleoside analogues using immobilized 2'-deoxyribosyltransferase from Lactobacillus reuteri, Appl Microbiol
Biotechnol, 91 (2011) 317-327.
[19] H.D.W. Hicks N., Synthesis of Nucleoside Analogues Using Immobilised N-Deoxyribosyltransferases,
Biocatalysis, 11 (1994) 1-7.
[20] U. Hanefeld, L. Gardossi, E. Magner, Understanding enzyme immobilisation, Chemical Society reviews, 38
(2009) 453-468.
[21] A. Fresco-Taboada, I. Serra, J. Fernandez-Lucas, C. Acebal, M. Arroyo, M. Terreni, I. De la Mata,
Nucleoside 2'-deoxyribosyltransferase from psychrophilic bacterium Bacillus psychrosaccharolyticus--preparation
of an immobilized biocatalyst for the enzymatic synthesis of therapeutic nucleosides, Molecules, 19 (2014)
11231-11249.
[22] W. John, The Protein Protocols Handbook, 3º ed., Humana Press2009.
[23] M.L. Brandon, L. Mi, W. Chaung, G. Teebor, R.J. Boorstein, 5-chloro-2'-deoxyuridine cytotoxicity results
from base excision repair of uracil subsequent to thymidylate synthase inhibition, Mutation research, 459 (2000)
161-169.
[24] T. Fujimaki, M. Matsutani, O. Nakamura, A. Asai, N. Funada, M. Koike, H. Segawa, K. Aritake, T.
Fukushima, S. Houjo, et al., Correlation between bromodeoxyuridine-labeling indices and patient prognosis in
cerebral astrocytic tumors of adults, Cancer, 67 (1991) 1629-1634.
[25] E.A. Véliz, P.A. Beal, 6-Bromopurine nucleosides as reagents for nucleoside analogue synthesis, The Journal
of organic chemistry, 66 (2001) 8592-8598.
14