TABLE 3 Real Sample Analysis
REFERENCES
NO2ꢁ ꢀ 108 ðmol Lꢁ1
Þ
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Sample
Added
Found
Well water
0
4.3 ꢂ 0.7
25.2 ꢂ 0.8
66.8 ꢂ 0.1
88.2 ꢂ 0.7
98.7 ꢂ 0.8
120.9 ꢂ 0.4
21.7
0
Rain water
River water
21.7
0
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21.7
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CONCLUSION
We demonstrated the interaction of 6-amino
coumarin (L) with nitrite ion in aqueous solution.
Structure of L was confirmed from single-crystal
X-ray structure analysis. L may be used as an efficient
and selective fluorescent sensor for trace level deter-
mination of nitrite ion in aqueous solution. L binds
very strongly to nitrite ion as evident from the signifi-
cant higher binding constant value. The method was
almost interference-free of other common accompa-
nying anions. Computational studies by DFT method
provided a molecular level interaction between
the reagent (L) and nitrite ion. The analysis of real
samples was carried out by this new method.
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method for the determination of nitrite in waters with pyridine-2-
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NOTES ON SUPPLEMENTARY DATA
CCDC 773331 contains the supplementary crystal-
lographic data for L. The data can be obtained free
retrieving.html or from the Cambridge Crystallo-
graphic Data Centre, 12 Union Road, Cambridge
CB2 1EZ, UK (fax (þ44) 1223- 336-033; or e-mail
deposit@ccdc.cam.ac.uk). Different parameters such
as bond moments, dipole moments, quadruple
moments, and optimized energies of the ligand L
ꢁ
and LþNO2 molecular complex are presented in
the supplementary data.
20. Murray, R. D. H.; Mendez, I.; Brawn, S. A. The Natural Coumarins;
Wiley: New York, 1983.
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El-Agrody, A. M. 4-Hydroxycoumarin in heterocyclic synthesis: Part
III. synthesis of some new pyrano[2,3-d]pyrimidine, 2-substitu-
ted[1,2,4]triazolo[1,5-c]pyrimidine and pyrimido[1,6-b][1,2,4]triazine
derivatives. ILFarmaco. 2000, 55, 708–714.
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marin derivatives as anti-fungal agents against soil-borne fungal
pathogens. Commun. Agric. Appl. Biol. Sci. 2007, 72(4), 785–793.
ACKNOWLEDGMENTS
The authors are grateful to IUC-DAE, Kolkata
Centre, and West Bengal Council of Science and
Technology (DST, Govt. of W. B.) for funding. Sisir
Lohar is grateful to CSIR, New Delhi, for providing
fellowship.
S. Guha et al.
232