Phytochemistry p. 359 - 362 (1989)
Update date:2022-08-29
Topics:
Katsumata, Teizo
Shige, Hiromitsu
Ejiri, Shin-Ichiro
Exogenously supplied 4-hydroxybenzoic acid (HBA) was converted quantitatively to 4-(β-D- glucopyranosyloxy)benzoic acid (HBA glucoside) during 30 hr of germination of the pollen of Pinus densiflora (pine pollen). <14C-carboxyl>HBA(<14C>-HBA) was also converted to <14C>-HBA glucoside by pine pollen.UDP glucose: 4-(β-D-glucopyranosyloxy)benzoic acid glucosyltransferase from pine pollen was assayed by measuring the rate of formation of <14C>-HBA glucoside from <14C>-HBA and UDPG.The enzyme was partially purified from the pine pollen for the first.The Mr, of the enzyme was estimated to be ca 33 000 by Sephacryl S-300 gel filtration.The enzyme had a pH optimum at 7.5, and its activity was increased ca 50 and 60percent by the addition of 3 mM Ca(2+) and 0.1 mM EDTA, respectively.The Km values for UDP glucose and 4-hydroxybenzoic acid were 2.4 x 1E-4 M and 2.9 x 1E-3 M, respectively.Key Word Index - Pinus densiflora; Pinaceae; Japanese red pine; pollen germination; pollen tube elongation; 4-hydroxybenzoic acid; 4-(β-D-glucopyranosyloxy)benzoic acid; UDPG; 4-(β-D-glucopyranosyloxy)benzoic acid glucosyltransferase
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