1794
P. Anitha et al.
6. Heffeter P, Bock K, Atil B, Hoda MAR, Korner W, Bartel C,
Jungwirth U, Keppler BK, Micksche M, Berger W, Koellen-
sperger GJ (2010) Biol Inorg Chem 15:737
The cells were grown in Dulbecco’s Modified Eagle’s
Medium containing 10 % fetal bovine serum (FBS). For
screening experiments, the cells were seeded into 96-well
plates in 100 mm3 of the respective medium containing
10 % FBS, at a plating density of 10,000 cells/well and
incubated at 37 °C, 5 % CO2, 95 % air, and 100 % relative
humidity for 24 h prior to the addition of the compounds.
The compounds were dissolved in DMSO (0.01 %) and
diluted in the respective medium containing 1 % FBS.
After 24 h, the medium was replaced with the respective
medium with 1 % FBS containing the compounds at var-
ious concentrations and incubated at 37 °C, 5 % CO2,
95 % air, and 100 % relative humidity for 48 h. Experi-
ments were performed in triplicate and the medium without
the compounds served as control. After 48 h, 15 mm3 of
MTT (5 mg cm-3) in phosphate buffered saline (PBS) was
added to each well and incubated at 37 °C for 4 h. The
medium with MTT was then removed and the formed
formazan crystals were dissolved in 100 mm3 of DMSO
and the absorbance measured at 570 nm using a micro
plate reader. The percentage cell inhibition was determined
using the following formula: % inhibition = [mean OD of
untreated cells (control)/mean OD of treated cells (con-
trol)] 9 100, and a graph was plotted of % of cell
inhibition versus concentration. From this plot, the IC50
value was calculated.
7. Kostova I (2006) Curr Med Chem 13:1085
8. Rademaker-Lakhai JM, Van Den Bongard D, Pluim D, Beijnen
JH, Schellens JHM (2004) Clin Cancer Res 10:3717
9. Hartinger CG, Zorbas-Seifried S, Jakupec MA, Kynast B, Zorbas
H, Keppler BK (2006) J Inorg Biochem 100:891
10. Jungwirth U, Kowol CR, Keppler BK, Hartinger CG, Berger W,
Heffeter P (2011) Antioxid Redox Signal 15:1085
11. Prabhakara MC, Basavaraju B, Bhojya Naik HS (2007) Bioinorg
Chem Appl 1:7
12. Vijayalakshmi R, Kanthimathi M, Parthasarathi R, Nair BU
(2006) Bioorg Med Chem 14:3300
13. Liang ZH, Li ZZ, Huang HL, Liu YJ (2011) J Coord Chem
64:3342
14. Driscoll JS, Hazard GF, Wood HB (1974) Cancer Chemother Rep
4:1
15. Lopes JN, Cruz FS, Docampo R (1978) Ann Trop Med Parasit
72:523
16. Li CJ, Zhang LJ, Dezubw BJ, Crumpacker CS, Pardee AB (1993)
Proc Natl Acad Sci U S A 90:1839
17. Li CJ, Wang C, Pardee AB (1955) Cancer Res 55:3712
18. Planchon SM, Wuerzberger S, Frydman B (1995) Cancer Res
55:3706
19. Li CJ, Averboukh I, Pardee AB (1993) J Biol Chem 268:22463
20. Boothman DA, Trask DK, Pardee AB (1989) Cancer Res 49:605
21. Frydman B, Marton LJ, Sun JS (1997) Cancer Res 57:620
22. Chen J, Huang YW, Liu G, Afrasiabi Z, Sinn E, Padhye S, Ma Y
(2004) Toxicol Appl Pharmacol 197:40
23. Afrasiabi Z, Sinn E, Kulkarni PP, Ambike V, Padhye S, Deo-
bagakar D, Heron M, Gabbutt C, Anson CE, Powell AK (2005)
Inorg Chim Acta 358:2023
24. Pal SN, Pal S (2002) J Chem Soc Dalton Trans 2102
25. Deepa KP, Aravindakshan KK (2000) Synth React Inorg Met Org
Chem 30:1601
Photoactivated DNA cleavage experiment
26. Borges RHU, Beraldo H, Abras A (1997) J Braz Chem Soc 8:33
27. Ulaganatha Raja M, Gowri N, Ramesh R (2010) Polyhdron
29:1175
28. Amoedo A, Grana M, Martinez J, Pereira T, Lopez-Torres M,
Fernandez A, Fernandez JJ, Vila JM (2002) Eur J Inorg Chem
613
29. El-Shahawi MS, Shoair AF (2004) Spectrochim Acta A 60:121
30. Samanta R, Mondal B, Munshi P, Lahiri GK (2001) J Chem Soc
Dalton Trans 1827
31. Joseph M, Sreekanth A, Suni V, Prathapachandra Kurup (2006)
Spectrochim Acta A 64:637
For the gel electrophoresis experiment, supercoiled pBR322
DNA (0.1 lg) was treated with the ruthenium(III) com-
plexes with buffer (50 mM Tris–HCl, 18 mM NaCl, pH 7.2)
and the solution was then irradiated at room temperature with
a UV lamp (365 nm, 10 W). The samples were analyzed by
electrophoresis for 1.5 h at 80 V on a 0.8 % agarose gel in
TBE (89 mM Tris–borate acid, 2 mM EDTA, pH 8.3). The
gel was stained with 1 lg cm-3 ethidium bromide and the
bands were observed under an illuminator.
32. Jayabalakrishnan C, Karvembu R, Natarajan K (2003) Synth
React Inorg Met Org Chem 33:1535
Acknowledgments The authors express their sincere thanks to the
Council of Scientific and Industrial Research (CSIR), New Delhi
[Grant No. 01(2437)/10/EMR-II] for financial support. One of the
authors (PA) thanks the CSIR for the award of Senior Research
Fellowship.
33. Heinze J (1984) Angew Chem Int Ed 23:831
34. Zhu NJ, Lecomte C, Coppens P, Keister JB (1982) Acta Cryst
38:1286
35. Farrugia LJ, Gillon AL, Grepioni F, Tedesco E (1999) Organo-
metallics 18:5022
36. Mathur P, Mavunkal IJ, Rugmini V, Mahon MF (1990) Inorg
Chem 29:4838
37. Masciocchi N, Moret M, Cairati P, Ragaini F, Sironi A (1993) J
Chem Soc Dalton Trans 471
References
38. Draper SM, Housecroft CE, Keep AK, Matthews DM, Song X
(1992) J Organomet Chem 423:241
1. Allardyce CS, Dyson PJ (2001) Platinum Met Rev 45:62
2. Clarke MJ (2003) Coord Chem Rev 236:209
39. Bast A, Haenen GRMM, Doelman CJA (1991) Am J Med 91:2
40. Beckford FA, Shaloski M, Leblanc G, Thessing J, Alleyne LCL,
Holder AA, Li L, Seeram NP (2009) Dalton Trans 10757
41. Xie YY, Huang HL, Yao JH, Lin GJ, Jiang GB, Liu YJ (2013)
Eur J Med Chem 63:603
3. Zhang CX, Lippard SJ (2003) Curr Opin Chem Biol 7:481
4. Ang WH, Dyson PJ (2006) Eur J Inorg Chem 4003
5. Groessl M, Reisner E, Hartinger CG, Eichinger R, Semenova O,
Timerbaev AR, Jakupec MA, Arion VB, Keppler BK (2007) J
Med Chem 50:2185
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