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RSC Advances
Page 4 of 5
DOI: 10.1039/C6RA21260C
COMMUNICATION
Journal Name
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Figure 4. TP confocal microscopy bright-field images (left) and
fluorescence images (middle) of rat liver frozen slice after
incubation with CBZ-H2O2 and CBZ-H2O2 + H2O2, respectively. And
3D TP confocal fluorescence images (right) of CBZ-H2O2 and CBZ-
H2O2 + H2O2 accumulated along the z direction at a depth of 0−40
μm and 0-105 μm with 40 × magnification, respectively.
6
N. Soh, O. Sakawaki, K. Makihara, Y. Odo, T. Fukaminato, T.
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Conclusions
Squirrell, D. L. Wokosin, J. G. White, B. D. Bavister. Nat.
Biotechnol., 1999, 17, 763.
In summary, we have designed and synthesized a new two-
photon fluorescent “turn-on” probe for mitochondrial H2O2
detection. The probe consists of a boronate ester functionality as a
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H. C. Heo, K. H. Kim, H. J. Kim, S. H. Baik, H. Song, Y. S. Kim, J.
sensing site,
a carbazole derivative as a fluorophore and a
triphenylphosphonium moiety as a mitochondrial-targeting localizer.
It exhibits not only fast response and high selectivity over other ROS,
RNS and related biomolecules but also low detection limit of 1.96
µM. Co-localization experiments clearly demonstrated that CBZ-
H2O2 selectively localizes to and accumulates in the mitochondria.
CBZ-H2O2 is also sensitive to both exogenous and endogenous H2O2.
By using two-photon fluorescence microscopy, it can detect H2O2 in
living cells and tissues at a depth of 105 μm for a long period of
time with excellent imaging quality. This probe will allow in-depth
investigation of the roles and functions of H2O2 in a complex
biological system.
Lee, I. Mook-jung, H. M. Kim, Chem. Commun., 2013, 49
,
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Anal. Chem., 2013, 85, 9877.
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Acknowledgements
Layton, C. Xu, Y. Chen, R. R. Ratan, J. Biomed. Opt., 2013, 18
106002.
,
This work was supported by the National Natural Science
Foundation of China (21305036, 21507135), Hunan Provincial
Natural Science Foundation of China (2015JJ3035), China
Postdoctoral Science Foundation funded project (2015M580685)
and Anhui Province Natural Science Foundation of China
(1608085MB30).
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4
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4 | J. Name., 2012, 00, 1-3
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